製品の概要

  • 製品名Human TIMP4 ELISA Kit
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 25 pg/ml
  • 検出範囲
    28.67 pg/ml - 7000 pg/ml
  • 添加回収試験

    80 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 96.11 89% - 104%
    Serum 73.49 67% - 82%
    Plasma 72.83 67% - 81%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s TIMP4 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TIMP4 in serum, plasma (collect plasma using EDTA and citrate as an anticoagulant. Heparin is not recommended), cell culture supernatants.

    This assay employs an antibody specific for Human TIMP4 coated on a 96- well plate. Standards and samples are pipetted into the wells and TIMP4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TIMP4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

法規制情報

製品の特性

  • 別名
    • Metalloproteinase inhibitor 4
    • TIMP 4
    • TIMP metallopeptidase inhibitor 4
    • Tissue inhibitor of metalloproteinase 4
    • Tissue inhibitor of metalloproteinases 4
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab113328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human TIMP4 ELISA Kit 画像

  • Representative Standard Curve using ab113328

  • Representative Standard Curve using ab113328

プロトコール

Human TIMP4 ELISA Kit (ab113328) 使用論文

ab113328 has not yet been referenced specifically in any publications.

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Thank you for your inquiry. Unofrtunately there is no straightforward way to categorize which kits can be used with heparin and which cannot. You need to look at the datasheet or contact us regarding the specific kit, as different manufacturers and rea...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"