製品の概要

  • 製品名Human TIMP1 ELISA Kit
    TIMP1 キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 40 pg/ml
  • 検出範囲
    24.69 pg/ml - 18000 pg/ml
  • 添加回収試験

    93 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 94.49 84% - 104%
    Serum 92.43 81% - 102%
    Plasma 93.67 83% - 102%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s TIMP1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TIMP1 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human TIMP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIMP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TIMP1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14.
  • 配列類似性Belongs to the protease inhibitor I35 (TIMP) family.
    Contains 1 NTR domain.
  • 翻訳後修飾The activity of TIMP1 is dependent on the presence of disulfide bonds.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • Clgi
    • Collagenase inhibitor
    • Collagenase inhibitor, Human
    • EPA
    • EPO
    • Erythroid Potentiating Activity
    • Erythroid-potentiating activity
    • Fibroblast collagenase inhibitor
    • FLJ90373
    • HCI
    • Human Collagenase Inhibitor
    • Metalloproteinase inhibitor 1
    • Metalloproteinase inhibitor 1 precursor
    • OTTHUMP00000023214
    • TIMP
    • TIMP 1
    • TIMP metallopeptidase inhibitor 1
    • TIMP-1
    • Timp1
    • TIMP1 protein
    • TIMP1_HUMAN
    • Tissue Inhibitor of Metalloproteinase 1
    • Tissue inhibitor of metalloproteinases
    • Tissue inhibitor of metalloproteinases 1
    • Ttissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100651 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human TIMP1 ELISA Kit 画像

  • Representative standard curve using ab100651

  • Representative standard curve using ab100651

プロトコール

Human TIMP1 ELISA Kit (ab100651) 使用論文

This product has been referenced in:
  • Natrajan MS  et al. Pioglitazone regulates myelin phagocytosis and multiple sclerosis monocytes. Ann Clin Transl Neurol 2:1071-84 (2015). ELISA ; Human . Read more (PubMed: 26734659) »

See 1 Publication for this product

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Thank you for contacting us.

You are correct!, this kit comes with 12 strips of 8 wells.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for your email. I am sorry for the delay while I was in contact with lab.

The volume of HRP-Streptavidin is very small and therefore would be difficult to see with the naked eye. The HRP-streptavidin volume sent was 8ul and is very ...

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Thank you for your inquiry and for forwarding your testing data. There are a few things I noticed after looking at the data: 1) The first thing I noticed was the background is quite high, ~0.5 OD. This high background is really reducing the dynamic ran...

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Thank you for contacting us. This kit ab100651 will detect free TIMP-1, both pro and active form (thus, total) however it is not known whether it will also detect the bound form (i.e. TIMP-MMP complexes).   I hope this information is helpful to you...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"