製品の概要

  • 製品名
    Human TIE2 ELISA Kit
    TIE2 キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Serum 6 7.1%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Serum 6 6.3%
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    0.03 ng/ml
  • 検出範囲
    0.16 ng/ml - 10 ng/ml
  • 添加回収試験

    87 %

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s TIE2 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human TIE2 concentrations in cell culture supernatant, serum and plasma (EDTA, citrate, heparin).

    TIE2 specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an biotinylated TIE2 detection antibody and the microplate is then incubated overnight at 4 ºC. Following washing with wash buffer a Streptavidin-HRP conjugate is added to each well, incubated at room temperature and then unbound conjugates are washed away using wash buffer. TMB is added and then catalyzed by HRP to produce a blue color product that changes into yellow after addition of an acidic stop solution. The density of yellow coloration is directly proportional to the amount of TIE2 captured on the plate.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

法規制情報

製品の特性

  • 保存方法
    Store at +4°C. Please refer to protocols.
  • 内容 1 x 96 tests
    20X Assay Buffer Concentrate 1 x 5ml
    20X Wash Buffer Concentrate 1 x 50ml
    Adhesive Films 4 units
    Biotin-Conjugate anti-human TIE-2 polyclonal antibody 1 x 70µl
    Human TIE2 Standard lyophilized 2 vials
    Polyclonal anti-Human TIE2 coated microplate (12 x 8 wells) 1 unit
    Sample Diluent 1 x 12ml
    Stop Solution (1M Phosphoric acid) 1 x 15ml
    Streptavidin-HRP 1 x 150µl
    TMB Substrate Solution 1 x 15ml
  • 研究分野
  • 機能
    This protein is a protein tyrosine-kinase transmembrane receptor for angiopoietin 1. It may constitute the earliest mammalian endothelial cell lineage marker. Probably regulates endothelial cell proliferation, differentiation and guides the proper patterning of endothelial cells during blood vessel formation.
  • 組織特異性
    Predominantly expressed in endothelial cells and their progenitors, the angioblasts. Has been directly found in placenta and lung, with a lower level in umbilical vein endothelial cells, brain and kidney.
  • 関連疾患
    Defects in TEK are a cause of dominantly inherited venous malformations (VMCM) [MIM:600195]; an error of vascular morphogenesis characterized by dilated, serpiginous channels.
  • 配列類似性
    Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily.
    Contains 3 EGF-like domains.
    Contains 3 fibronectin type-III domains.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 翻訳後修飾
    Phosphorylated. Phosphorylation is induced by ANGPT1 and ANGPT2. ANGPT1-induced phosphorylation is impaired during hypoxia. Dephosphorylated by PTPRB.
    Autophosphorylation at Tyr-1108 is critical for coupling downstream cell migration signal transduction pathways with ANG1 stimulation in endothelial cells.
  • 細胞内局在
    Membrane.
  • Information by UniProt
  • 別名
    • Angiopoietin-1 receptor
    • CD202b
    • CD202b antigen
    • EC 2.7.10.1
    • hTIE2
    • p140 TEK
    • soluble TIE2 variant 1
    • soluble TIE2 variant 2
    • Tek
    • TEK tyrosine kinase, endothelial
    • TIE 2
    • TIE2_HUMAN
    • Tunica interna endothelial cell kinase
    • Tyrosine-protein kinase receptor TEK
    • Tyrosine-protein kinase receptor TIE 2
    • Tyrosine-protein kinase receptor TIE-2
    • venous malformations, multiple cutaneous and mucosal
    • VMCM
    • VMCM1
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab119559 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative Standard Curve using ab119559.

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参考文献

ab119559 has not yet been referenced specifically in any publications.

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