製品の概要
- 製品名Human TIE2 ELISA Kit
TIE2 キット 製品一覧 - 検出方法Colorimetric
- サンプルの種類Cell culture supernatant, Serum, Plasma
- アッセイタイプSandwich (quantitative)
- 検出感度< 20 pg/ml
- 検出範囲20.58 pg/ml - 15000 pg/ml
- 添加回収試験
97 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 76.59 68% - 92% Serum 110.9 101% - 118% Plasma 104.2 93% - 118% - ステップMultiple steps standard assay
- 種交差性交差種: Human
- 製品の概要
Abcam’s TIE2 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TIE2 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human TIE2 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIE2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TIE2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIE2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- 特記事項
Optimization may be required with urine samples.
- アプリケーション適用あり: Sandwich ELISAmore details
- 試験プラットフォームMicroplate
製品の特性
- 保存方法Store at -20°C. Please refer to protocols.
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内容 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human TIE2 2 vials Recombinant Human TIE2 Standard 2 vials Stop Solution 1 x 8ml TIE2 Microplate (12 x 8 wells) 1 unit TMB One-Step Substrate Reagent 1 x 12ml - 研究分野
- 機能This protein is a protein tyrosine-kinase transmembrane receptor for angiopoietin 1. It may constitute the earliest mammalian endothelial cell lineage marker. Probably regulates endothelial cell proliferation, differentiation and guides the proper patterning of endothelial cells during blood vessel formation.
- 組織特異性Predominantly expressed in endothelial cells and their progenitors, the angioblasts. Has been directly found in placenta and lung, with a lower level in umbilical vein endothelial cells, brain and kidney.
- 関連疾患Defects in TEK are a cause of dominantly inherited venous malformations (VMCM) [MIM:600195]; an error of vascular morphogenesis characterized by dilated, serpiginous channels.
- 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily.
Contains 3 EGF-like domains.
Contains 3 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. - 翻訳後修飾Phosphorylated. Phosphorylation is induced by ANGPT1 and ANGPT2. ANGPT1-induced phosphorylation is impaired during hypoxia. Dephosphorylated by PTPRB.
Autophosphorylation at Tyr-1108 is critical for coupling downstream cell migration signal transduction pathways with ANG1 stimulation in endothelial cells. - 細胞内局在Membrane.
- Information by UniProt
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別名
- Angiopoietin-1 receptor
- CD202b
- CD202b antigen
see all -
参照データベース
- Entrez Gene: 7010 Human
- SwissProt: Q02763 Human
- Unigene: 89640 Human
アプリケーション
Our Abpromise guarantee covers the use of ab100650 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Human TIE2 ELISA Kit 画像
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Typical standard curveRepresentative standard curve using ab100650
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Typical standard curveRepresentative standard curve using ab100650
プロトコール
Human TIE2 ELISA Kit (ab100650) 使用論文
ab100650 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"