97
%
サンプルの種類 | 平均 % | 測定範囲 |
---|---|---|
Cell culture supernatant | 76.59 | 68% - 92% |
Serum | 110.9 | 101% - 118% |
Plasma | 104.2 | 93% - 118% |
Abcam’s TIE2 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TIE2 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human TIE2 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIE2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TIE2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIE2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
内容 | 1 x 96 tests |
---|---|
20X Wash Buffer Concentrate | 1 x 25ml |
300X HRP-Streptavidin Concentrate | 1 x 200µl |
5X Assay Diluent B | 1 x 15ml |
5X Assay Diluent D | 1 x 15ml |
Assay Diluent A | 1 x 30ml |
Biotinylated anti-Human TIE2 | 2 vials |
Recombinant Human TIE2 Standard | 2 vials |
Stop Solution | 1 x 8ml |
TIE2 Microplate (12 x 8 wells) | 1 unit |
TMB One-Step Substrate Reagent | 1 x 12ml |
Our Abpromise guarantee covers the use of ab100650 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Sandwich ELISA | Use at an assay dependent concentration. |
ab100650 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"