製品の概要

  • 製品名
    Human Thyroxine ELISA Kit (T4)
    Thyroxine (T4) キット 製品一覧
  • 検出方法
    Colorimetric
  • サンプルの種類
    Serum
  • アッセイタイプ
    Competitive
  • 検出感度
    = 0.4 µg/dl
  • 検出範囲
    0.4 µg/dl - 25 µg/dl
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    ab108661, Thyroxine (T4) Human ELISA Kit is intended for the quantitative determination of Total Thyroxine (T4) concentration in Human serum.


    L-Thyroxine (T4) is a hormone that is synthesized and stored in the thyroid gland. Proteolytic cleavage of follicular thyroglobulin releases T4 into the bloodstream. Greater than 99% of Thyroxine (T4) is reversibly bound to three plasma proteins in blood - thyroxine binding globulin (TBG) binds 70%, thyroxine binding pre-albumin (TBPA) binds 20%, and albumin binds 10%. Approximately 0.03% of T4 is in the free, unbound state in blood at any one time.

  • アプリケーション
    適用あり: ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108661 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent dilution.

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  • Thyroxine (T4) Standard Curve

プロトコール

参考文献

ab108661 has not yet been referenced specifically in any publications.

レビューと Q&A

Abreviews
Thyroid tissue was maintained within a microfluidic culture device, continually perfused with medium. The media effluent coming from the device was tested for the presence of thyroxine as a potential marker of tissue viability/ activity. Although the ELISA was not advertised as validated for cell culture supernatant samples, we endevoured to determine its use in this system. Unfortunately, although the ELISA produced a good standard curve, the results obtained from the samples were not ideal as the medium only controls contained a high threshold of thyroxine - presumably due to bovine thyroxine within the FBS which supplemented the medium. A possible work-around which we are looking into is charcoal stripping the serum before incorporating into the media, in order to remove bovine thyroxine before re-running the ELISA.
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Andrew Riley

Verified customer

投稿 Feb 20 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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