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ab108662, Thyroxine (fT4) Human ELISA Kit is intended for the quantitative determination of Free Thyroxine (fT4) concentration in Human serum.
Thyroxine (T4) or 3,5,3’,5’-tetraiodothyronine is the most commonly measured thyroid hormone for the diagnosis of thyroid function. Thyroxine (T4) has its primary infuence on protein synthesis and oxygen consumption in virtually all tissues but it is also important for growth, development, and sexual maturation. Thyroxine (T4) is synthesized by the thyroid gland and is secreted into the bloodstream. Here the Thyroxine (T4) becomes bound to serum proteins for transport to the cells. The major transport protein is Thyroxine Binding Globulin (TBG) which normally accounts for 80% of the bound Thyroxine (T4). Other thyroid hormone binding proteins are Thyroxine Binding Prealbumin and Albumin. Most of the serum Thyroxine (T4) is bound to these transport proteins leaving only about 0.03% free to exert its effect on cells. It is the free Thyroxine (T4) (fT4) that represents the metabolically active fraction; for this reason the measurement of fT4 concentration is considered to be an indicator of patient thyroid status. Primary hypothyroidism results in underproduction of Thyroxine (T4) by the thyroid gland and consequently an abnormally low circulating fT4 concentration in the blood. Primary hyperthyroidism leads to excessive thyroid production on Thyroxine (T4) and resulting elevated fT4 concentration.
|内容||1 x 96 tests|
|Color Reagent A||1 x 13ml|
|Color Reagent B||1 x 13ml|
|Free Thyroxine Standard 1 - 0ng/dL||1 x 1ml|
|Free Thyroxine Standard 2 - 0.3 ng/dL||1 x 1ml|
|Free Thyroxine Standard 3 - 0.95ng/dL||1 x 1ml|
|Free Thyroxine Standard 4 - 2.1ng/dL||1 x 1ml|
|Free Thyroxine Standard 5 - 3.6ng/dL||1 x 1ml|
|Free Thyroxine Standard 6 - 7.0ng/dL||1 x 1ml|
|Stop solution||1 x 10ml|
|T4 Antibody-Coated Microplate, 96 wells||1 x 96 packs|
|Thyroxine (T4)-Enzyme Conjugate Reagent||1 x 10.5ml|
Our Abpromise guarantee covers the use of ab108662 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Competitive ELISA||Use at an assay dependent dilution.|
ab108662 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"