製品の概要

  • 製品名Human Thyroxine ELISA Kit (Free T4)
    Thyroxine (T4) キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Serum, Plasma
  • アッセイタイプCompetitive
  • 検出感度
    0.5 pg/ml
  • 添加回収試験

    97.8 %

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Thyroxine (T4) in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Thyroxine in Human serum and plasma.

     

    A 96-well plate has been precoated with anti-Thyroxine antibodies. Samples and the Thyroxine-HRP conjugate are added to the wells, where Thyroxine in the sample competes with the added
    Thyroxine-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Thyroxine in the sample and the intensity is measured at 450 nm.

     

  • アプリケーション適用あり: Competitive ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Competitive ELISA Use at an assay dependent dilution.

Human Thyroxine ELISA Kit (Free T4) 画像

  • Representative Standrad Curve using ab108686.

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Human Thyroxine ELISA Kit (Free T4) (ab108686) 使用論文

ab108686 has not yet been referenced specifically in any publications.

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