Human Thrombomodulin ELISA Set (without plates) (ab47358)

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製品の概要

  • 製品名
    Human Thrombomodulin ELISA Set (without plates)
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 0.38 ng/ml
  • 検出範囲
    0.625 ng/ml - 20 ng/ml
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Thrombomodulin Human in vitro ELISA Set is designed for the quantitative measurement of Thrombomodulin in cell culture supernatants, buffered solutions, serum, plasma and other body fluids.

     

    This ELISA Set is intended to measure human Thrombomodulin in cell -culture supernatants, as well as in serum and plasma samples. The enclosed procedures provide general guidelines for ELISA preparation. This procedure may need some optimization depending on the plastic, matrix, standard range.

    These reagents are intended for research use only, not for diagnostic purposes.

     

    The Thrombomodulin ELISA Set includes both the capture and the detection antibodies as well as Horse-Radish peroxidase conjugated Streptavidin (HRP-Strep) and TMB which are the reagents used in the colour development step.

     

    Recognizes both natural and recombinant human Thrombomodulin.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Reagents

製品の特性

  • 保存方法
    Store at +4°C. Please refer to protocols.
  • 内容 5 x 96 tests 10 x 96 tests 15 x 96 tests
    Detection biotinylated anti-Thrombomodulin antibody 1 x 0.55ml 2 x 0.55ml 3 x 0.55ml
    Chromogen TMB Substrate Solution 2 vials 4 vials 6 vials
    Streptavidin-HRP 1 vial 2 vials 3 vials
    Thrombomodulin Capture Antibody 1 x 0.1ml 2 x 0.1ml 3 x 0.1ml
    Thrombomodulin standard 5 vials 10 vials 15 vials
  • 研究分野
  • 関連性
    Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. Endothelial cells are unique in synthesizing thrombomodulin. The thrombomodulin/thrombin complex is responsible for the conversion of protein C to the activated protein C (protein Ca), which degrades clotting factors Va and VIIIa and reduces the amount of thrombin generated. Defects in Thrombomodulin may be a cause of thromboembolic disease.
  • 細胞内局在
    single-pass type I membrane protein
  • 別名
    • CD141
    • CD141 antigen
    • Fetomodulin
    • THBD
    • THRM
    • TM
    see all
  • 参照データベース

    アプリケーション

    Our Abpromise guarantee covers the use of ab47358 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Sandwich ELISA Use at an assay dependent concentration.

    画像

    • Thrombomodulin Human ELISA Set (ab47358)
      Thrombomodulin Human ELISA Set (ab47358)
      Thrombomodulin Standard curve ranging from 0.625 to 20 ng/ml

    参考文献

    ab47358 has not yet been referenced specifically in any publications.

    ab47358 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

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    1 Review or Q&A

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    what kind of protein is ab47358 standard? from human or recombinent protein? If it is recombinent protein, would you please help us to check the sequence? (fragment or full length?)

    Thank you for contacting us. The standard protein in the kit ab47358 is a recombinant human thrombomodulin, carboxyl-terminal truncated form - lacks the putative transmembrane and the cytoplasmic domains (~38 amino acids) - purified from a Chinese ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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