製品の概要

  • 製品名Human Thrombin ELISA Kit (Factor II)
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4.7%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 7.2%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    = 0.3 ng/ml
  • 検出範囲
    0.313 ng/ml - 20 ng/ml
  • 添加回収試験

    98 %

  • 全工程の試験時間
    4h 0m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Thrombin (Factor II) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Thrombin concentrations in cell culture supernatants. This assay recognizes both natural and recombinant Human thrombin.

    A Thrombin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Thrombin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Thrombin captured in plate.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108909 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent dilution.

Human Thrombin ELISA Kit (Factor II) 画像

  • Representative Standard Curve Using ab108909.

プロトコール

Human Thrombin ELISA Kit (Factor II) (ab108909) 使用論文

This product has been referenced in:
  • Liu JF  et al. Thrombin induces heme oxygenase-1 expression in human synovial fibroblasts through protease-activated receptor signaling pathways. Arthritis Res Ther 14:R91 (2012). Sandwich ELISA ; Human . Read more (PubMed: 22541814) »

See 1 Publication for this product

Product Wall

The thrombin ELISA, using the Abcam human ELISA kit Ab108909, lot GR232366-8, was performed by an experienced laboratory technician at an accredited university. The goal of the experiment was to analyze the linearity of dilutions of rhesus macaque EDTA-prepared plasma samples and serum samples.

Overall, the kit, like other Abcam ELISA kits, was easy to follow, clear in terms of directions, and brief yet thorough. The laboratory technician had no problems understanding any of the protocol’s steps.

Our test was performed in 2 basic steps. The first was to find the appropriate testable dilution in plasma and serum, and the second was to test the linearity and spike recovery.

To this end our first experiment tested 8 dilutions of both serum and plasma ranging from 1:10 to 1:163840 (8 1:4 serial dilutions). The plasma was not in range of the assay, confirming that his kit does not detect pro-thrombin. The serum dilutions gave OD values in range of the assay from 1:10 – 1:2560, but a linear range was not observed.

We saved the thrombin standard vial at -80C for 1 week between tests.

Our second experiment consisted of a narrower dilution range at 1:50, 1:100, 1:200, 1:400,a nd 1:800 for n=2 rhesus sera and a spike recovery test. The tested range was well within the OD limits of the assay. N=1 rhesus serum may have been near linear range at 1:400-1:800, but n=1 rhesus serum was not. The spike recovery (performed with Serum from animal 2) showed an increasing spike recovery with dilution (40% at 1:50, 80% at 1:800).

Overall, we would recommend this kit for its ease of use. We believe there is positive cross-reactivity for rhesus, but we were unable to identify a linear range for the assay.
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投稿 Jun 07 2016

Thank you for your patience while I looked into this.

The lab has sent us thereplacement vials of the diluent and wash buffer. We are sending these to you free of charge on order. You should receive these tomorrow.

I hope this infor...

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Thank you for contacting Abcam regarding ab108909.


Just to confirm what we discussed over the phone:


Thrombin Standard: Purified human Thrombin in a buffered protein base (40 ng, lyophilized).

Standard Curve: Recons...

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Thank you for contacting us.

I heard back from the lab and they recommend that youdispose the Wash Buffer and the Diluent if you mixed them together.

Wash Buffer is 20x and should be diluted with water (1 part Wash Buffer + 19 parts...

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Thank you for calling Abcam earlier today.
I have talked to the lab and they do not think there is protein the same size as thrombin in the buffer based, but we cannot be 100% sure. The exact components of the buffer are proprietary and so I am una...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"