製品の概要

  • 製品名Human TGF beta 2 ELISA Kit
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 15 pg/ml
  • 検出範囲
    8.23 pg/ml - 6000 pg/ml
  • 添加回収試験

    95 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 96.14 84% - 105%
    Serum 94.74 82% - 104%
    Plasma 95.53 83% - 103%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s TGF beta 2 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TGF beta 2 in serum, plasma and cell culture supernatants

    This assay employs an antibody specific for Human TGF beta 2 coated on a 96-well plate. Standards and samples are pipetted into the wells and TGF beta 2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TGF beta 2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TGF beta 2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能TGF-beta 2 has suppressive effects on interleukin-2 dependent T-cell growth.
  • 関連疾患Note=A chromosomal aberration involving TGFB2 is found in a family with Peters anomaly. Translocation t(1;7)(q41;p21) with HDAC9.
    Defects in TGFB2 are the cause of Loeys-Dietz syndrome 4 (LDS4) [MIM:614816]. An aortic aneurysm syndrome with widespread systemic involvement. LDS4 is characterized by arterial tortuosity, aortic dissection, intracranial aneurysm and subarachnoid hemorrhage, hypertelorism, bifid uvula, pectus deformity, bicuspid aortic valve, arachnodactyly, scoliosis, foot deformities, dural ectasia, joint hyperflexibility, and thin skin with easy bruising and striae.
  • 配列類似性Belongs to the TGF-beta family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • BSC 1 cell growth inhibitor
    • BSC-1 cell growth inhibitor
    • Cetermin
    • G-TSF
    • Glioblastoma derived T cell suppressor factor
    • Glioblastoma-derived T-cell suppressor factor
    • Polyergin
    • TGF beta2
    • TGF-beta-2
    • TGFB2
    • TGFB2_HUMAN
    • Transforming growth factor beta-2
    • Transforming growth factor, beta 2
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human TGF beta 2 ELISA Kit 画像

  • Representative standard curve using ab100648

  • Representative standard curve using ab100648

プロトコール

Human TGF beta 2 ELISA Kit (ab100648) 使用論文

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