製品の概要

  • 製品名Human TGF beta 1 ELISA Kit
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 3.1%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 7.6%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    = 0.03 ng/ml
  • 検出範囲
    0.031 ng/ml - 2 ng/ml
  • 全工程の試験時間
    5h 00m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s TGF beta 1 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of TGF beta 1 concentrations in cell culture supernatants.

    A TGF beta 1 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a TGF beta 1 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of TGF beta 1 captured in plate. This assay recognizes both natural and recombinant Human TGF beta 1.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
  • 組織特異性Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Colocalizes with ASPN in chondrocytes within OA lesions of articular cartilage.
  • 関連疾患Camurati-Engelmann disease
  • 配列類似性Belongs to the TGF-beta family.
  • ドメインThe 'straitjacket' and 'arm' domains encircle the growth factor monomers and are fastened together by strong bonding between Lys-56 and Tyr-103/Tyr-104. Activation of TGF-beta1 requires the binding of integrin alpha-V to an RGD sequence in the prodomain and exertion of force on this domain, which is held in the extracellular matrix by latent TGF-beta binding proteins. The sheer physical force unfastens the straitjacket and releases the active growth factor dimer.
  • 翻訳後修飾Glycosylated.
    The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive.
  • 細胞内局在Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 別名
    • LAP
    • Latency-associated peptide
    • TGF-beta-1
    • tgfb1
    • TGFB1_HUMAN
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab108912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent dilution.

Human TGF beta 1 ELISA Kit 画像

  • Representative Standard Curve using ab108912.

プロトコール

Human TGF beta 1 ELISA Kit (ab108912) 使用論文

ab108912 has not yet been referenced specifically in any publications.

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