製品の概要

  • 製品名Human TGF alpha ELISA Kit
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 3 pg/ml
  • 検出範囲
    1.37 pg/ml - 1000 pg/ml
  • 添加回収試験

    93 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 94.49 84% - 105%
    Serum 92.34 82% - 104%
    Plasma 93.46 83% - 103%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s TGF alpha Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TGF alpha in serum, plasma, cell culture supernatants

    This assay employs an antibody specific for Human TGF alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and TGF alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TGF alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TGF alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 関連性Transforming growth factor alpha (TGF alpha) is an acid- and heat-stable originally discovered in rodents and humans. TGF alpha protien is 33% homologous to epidermal growth factor (EGF). TGF alpha binds to the EGF receptor, mediating tyrosine phosphorylation of the receptor, and promotes anchorage-independent growth of normal rat fibroblasts in soft agar in the presence of transforming growth factor-beta. TGF alpha is secreted by a variety of transformed cells and tumors, embryonic cells and some normal adult cells . TGF alpha bioactivity has also been found in the urine of cancer patients. It has been suggested that it may act as an autocrine growth factor for the induction or maintenance of malignancy.
  • 別名
    • EGF like TGF
    • ETGF
    • OTTHUMP00000203300
    • OTTHUMP00000203301
    • OTTHUMP00000203345
    • OTTHUMP00000203352
    • Protransforming growth factor alpha
    • RATTGFAA
    • TFGA
    • TGF A
    • TGF type 1
    • TGFA
    • Transforming growth factor alpha
    • Transforming growth factor alpha precursor
    • Wa1
    • Waved 1
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100646 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human TGF alpha ELISA Kit 画像

  • Representative standard curve using ab100646

  • Representative standard curve using ab100646

プロトコール

Human TGF alpha ELISA Kit (ab100646) 使用論文

This product has been referenced in:
  • Mouradian M  et al. Key roles for GRB2-associated-binding protein 1, phosphatidylinositol-3-kinase, cyclooxygenase 2, prostaglandin E2 and transforming growth factor alpha in linoleic acid-induced upregulation of lung and breast cancer cell growth. Prostaglandins Leukot Essent Fatty Acids 90:105-15 (2014). Read more (PubMed: 24374147) »
  • Tukaj S  et al. Aberrant expression and secretion of heat shock protein 90 in patients with bullous pemphigoid. PLoS One 8:e70496 (2013). Read more (PubMed: 23936217) »

See all 2 Publications for this product

Product Wall

Thank you for your interest in our ELISA kits. Each kit comes with a microplate that is already coated with an antibody specific for the the protein of interest, so you would not be able to use one plate to detect all three proteins (EGF, TGF, betacell...

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Merci de nous avoir contactés.

Le kit ELISA ab100646 est en effet approprié pour le dosage du TGF-alpha humain contenu dans les supernageants de cultures cellulaires, urine, sérum et plasma.

Pour les anti-Histone ...

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Abreview for TGF alpha Human ELISA Kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews

Sample: Human Serum (serum)
Specification: serum
Amount of Sample: 20 uL µg
Type of Kit Used: ELISA
Positive control: We did not measure positive control except for kit standards.
Did you use the Abcam Kit protocol?: Yes
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投稿 Aug 03 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"