製品の概要

  • 製品名Human TARC ELISA Kit
    TARC キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 5 pg/ml
  • 検出範囲
    4.1 pg/ml - 1000 pg/ml
  • 添加回収試験

    87 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 106.7 98% - 122%
    Serum 76.75 70% - 93%
    Plasma 78.32 73% - 97%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s TARC Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TARC in serum, plasma and cell culture supernates.

    This assay employs an antibody specific for Human TARC coated on a 96-well plate. Standards and samples are pipetted into the wells and TARC present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TARC antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TARC bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Chemotactic factor for T-lymphocytes but not monocytes or granulocytes. May play a role in T-cell development in thymus and in trafficking and activation of mature T-cells. Binds to CCR4.
  • 組織特異性Expressed at high levels in thymus and at low levels in the lung, colon and small intestine.
  • 配列類似性Belongs to the intercrine beta (chemokine CC) family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • A 152E5.3
    • ABCD 2
    • C C motif chemokine 17
    • C-C motif chemokine 17
    • CC chemokine TARC
    • CCL 17
    • CCL17
    • CCL17_HUMAN
    • Chemokine (C C motif) ligand 17
    • Chemokine ligand 17
    • MGC138271
    • MGC138273
    • SCYA17
    • Small inducible cytokine A17
    • Small inducible cytokine subfamily A (Cys Cys) member 17
    • Small inducible cytokine subfamily A member 17
    • Small-inducible cytokine A17
    • T cell directed CC chemokine
    • Thymus and activation regulated chemokine
    • Thymus and activation-regulated chemokine
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100644 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human TARC ELISA Kit 画像

  • Representative standard curve using ab100644

プロトコール

Human TARC ELISA Kit (ab100644) 使用論文

ab100644 has not yet been referenced specifically in any publications.

Product Wall

CCL17 secretion by human macrophages

Excellent Excellent 5/5 (Ease of Use)
Abreviews
CCL17 secretion by human macrophages isolated by negative selection in culture for 3 and 7 days.
Kit easy to use, the assay takes about 5.30hr, excellent specificity as shown by higher secretion by M2a macrophages (MSCF+IL4) on day 7 compared to the others subtypes (M0=MCSF M1=MSCF+IFNg DEX=MCSF+Dexamethason IL10=MCSF+IL10 TGFb=MCSF+TGFb)
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Abcam user community

Verified customer

投稿 Apr 01 2014

Thanks for your inquiry. Based upon sequence homology, I would not expect any of the antibodies to cross react with mouse as there is only a 64% homology. Please let me know if you require further assistance.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"