Human TACE ELISA Kit (ADAM17) (ab113321)
製品の概要
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製品名Human TACE ELISA Kit (ADAM17)
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検出方法Colorimetric
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サンプルの種類Cell culture supernatant, Serum, Plasma
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アッセイタイプSandwich (quantitative)
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検出感度< 70 pg/ml
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検出範囲78.15 pg/ml - 5000 pg/ml
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添加回収試験
100 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 98.65 89% - 124% Serum 112.3 85% - 132% Plasma 101.1 72% - 128% -
ステップMultiple steps standard assay
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種交差性交差種: Human
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製品の概要
Abcam’s TACE Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TACE in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human TACE coated on a 96-well plate. Standards and samples are pipetted into the wells and TACE present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TACE antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TACE bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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特記事項
Optimization may be required with urine samples.
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アプリケーション適用あり: Sandwich ELISAmore details
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試験プラットフォームMicroplate
法規制情報
製品の特性
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保存方法Store at -20°C. Please refer to protocols.
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内容 ラベル 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated anti-Human TACE Item F 2 vials Recombinant Human TACE Standard 2 vials Stop Solution 1 x 8ml TACE Microplate (12 x 8 wells) Item A 1 unit TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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参照データベース
- SwissProt: P78536 Human
アプリケーション
Our Abpromise guarantee covers the use of ab113321 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
画像
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Typical Standard CurveRepresentative standard curve using ab113321
プロトコール
参考文献
This product has been referenced in:
- Erdoes G et al. CD62L (L-selectin) shedding for assessment of perioperative immune sensitivity in patients undergoing cardiac surgery with cardiopulmonary bypass. PLoS One 8:e53045 (2013). ELISA ; Human . Read more (PubMed: 23301018) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"