Human TACE ELISA Kit (ADAM17) (ab113321)

製品の概要

• 製品名
  Human TACE ELISA Kit (ADAM17)
• 検出方法
  Colorimetric
• サンプルの種類
  Cell culture supernatant, Serum, Plasma
• アッセイタイプ
  Sandwich (quantitative)
• 検出感度
  < 70 pg/ml
• 検出範囲
  78.15 pg/ml - 5000 pg/ml
• 添加回収試験

  100 %
  

  特定サンプルでの回収試験

  サンプルの種類	平均 %	測定範囲
  Cell culture supernatant	98.65	89% - 124%
  Serum	112.3	85% - 132%
  Plasma	101.1	72% - 128%

• ステップ
  Multiple steps standard assay
• 種交差性
  交差種: Human
  
• 製品の概要

  Abcam’s TACE Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TACE in serum, plasma and cell culture supernatants.

  This assay employs an antibody specific for Human TACE coated on a 96-well plate. Standards and samples are pipetted into the wells and TACE present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TACE antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TACE bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

• 特記事項

  Optimization may be required with urine samples.

• アプリケーション
  適用あり: Sandwich ELISAmore details
• 試験プラットフォーム
  Microplate

製品の特性

• 保存方法
  Store at -20°C. Please refer to protocols.

• 内容	ラベル	1 x 96 tests
  200X HRP-Streptavidin Concentrate		1 x 200µl
  20X Wash Buffer Concentrate		1 x 25ml
  5X Assay Diluent B	Item E	1 x 15ml
  Assay Diluent C	Item L	1 x 30ml
  Biotinylated anti-Human TACE	Item F	2 vials
  Recombinant Human TACE Standard		2 vials
  Stop Solution		1 x 8ml
  TACE Microplate (12 x 8 wells)	Item A	1 unit
  TMB One-Step Substrate Reagent		1 x 12ml

• 研究分野
  • Cell Biology
  • Apoptosis
  • Receptors
  • Associated Proteins

  • Cell Biology
  • Cell Cycle
  • Cell differentiation

  • Signal Transduction
  • Cytoskeleton / ECM
  • Extracellular Matrix
  • ECM Enzymes
  • ADAM Protein Family

  • Cancer
  • Invasion/microenvironment
  • ECM
  • Extracellular matrix
  • ADAM protein family

  • Cell Biology
  • Proteolysis / Ubiquitin
  • Proteolytic enzymes
  • Metalloprotease
  • ADAMs

  • Kits/ Lysates/ Other
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  • ELISA Kits
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  • TNF ELISA kits

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  • Cancer proteins ELISA kits

  • Kits/ Lysates/ Other
  • Kits
  • ELISA Kits
  • ELISA Kits
  • Protease inhibitors ELISA kits

  • Cancer
  • Cell Death
  • Apoptosis
  • Receptors
  • Associated Proteins

Human TACE ELISA Kit (ADAM17) 画像

• 

  Typical Standard Curve

  Representative standard curve using ab113321