製品の概要

  • 製品名Human Syndecan-1 ELISA Kit (CD138)
    Syndecan-1 キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 6 6.2%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 6 10.2%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    = 4.94 ng/ml
  • 検出範囲
    8 ng/ml - 256 ng/ml
  • 添加回収試験

    101 %

  • 全工程の試験時間
    1h 45m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Syndecan-1 (CD138) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Syndecan-1 in Human serum, plasma, buffered solutions and supernatants.

    A monoclonal antibody specific for Syndecan-1 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known Syndecan-1 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for Syndecan-1 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of Syndecan-1 present in the samples.

    This kit will recognize both endogenous and recombinant Human Syndecan-1.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

法規制情報

製品の特性

  • 機能Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix.
  • 配列類似性Belongs to the syndecan proteoglycan family.
  • 翻訳後修飾Shedding is enhanced by a number of factors such as heparanase, thrombin or EGF. Also by stress and wound healing. PMA-mediated shedding is inhibited by TIMP3.
  • 細胞内局在Membrane. Secreted. Shedding of the ectodomain produces a soluble form.
  • Information by UniProt
  • 別名
    • CD138
    • CD138 antigen
    • SDC
    • Sdc1
    • SDC1_HUMAN
    • SYND1
    • Syndecan 1
    • Syndecan-1
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab46506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human Syndecan-1 ELISA Kit (CD138) 画像

  • Syndecan measured in undiluted cell culture supernatants, including the positive control from kit (duplicates +/- SD).

  • Syndecan measured in human biological fluids (dilution range 1/1-1/10); mouse and pig samples (plasma and serum) were below limit of detection (duplicates +/- SD).

  • Standard curve of human syndecan with background signal subtracted (duplicates; +/- SD).

  •  Representative Standard Curve using ab46506

プロトコール

Human Syndecan-1 ELISA Kit (CD138) (ab46506) 使用論文

This product has been referenced in:
  • Rahbar E  et al. Endothelial glycocalyx shedding and vascular permeability in severely injured trauma patients. J Transl Med 13:117 (2015). Read more (PubMed: 25889764) »

See 1 Publication for this product

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Thank you for your call earlier this week and for your patience while I've been in touch with the lab regarding your enquiry.

The control provided in the kit is the same protein as the standard, and they are both native purified protein isola...

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Thank you for contacting us.

In order to measure syndecan-1 from cell culture supernatants, spinning at 1000g during 10 minutes is necessary to remove particulates and aggregates.

How was the thaw process performed? Was it by heatin...

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ab46506, CD138 ELISA kit Antibodies are raised in mouse.

I hope this helps. Please contact me again if you have any further questions.

Thank you for your enquiry. The standard used is natural protein coming from U266 cells supernatent (unpurified).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"