製品の概要

  • 製品名
    Human Superoxide Dismutase 1 ELISA Kit
    Superoxide Dismutase 1 キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 5.1%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 5.8%
  • サンプルの種類
    Cell culture supernatant, Urine, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    0.04 ng/ml
  • 検出範囲
    0.08 ng/ml - 5 ng/ml
  • 添加回収試験

    98 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Serum 98 89% - 108%

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Human Superoxide Dismutase 1 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human Cu/Zn Superoxide Dismutase concentrations in cell culture supernatant, serum and plasma (EDTA, citrate, heparin), amniotic fluid, fetal umbilical vein blood and urine.


    Cu/Zn Superoxide Dismutase specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells along with a Cu/Zn Superoxide Dismutase HRP-conjugated detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is added and catalyzed by HRP to produce a blue color product that changes to yellow after addition of an acidic stop solution. The density of yellow coloration is directly proportional to the Cu/Zn Superoxide Dismutase amount of sample captured in plate.

  • 特記事項

    ab119520 Human Superoxide Dismutase 1 ELISA kit has been shown to detect both SOD1 and SOD3.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab119520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Representative Standard Curve using ab119520.

プロトコール

参考文献

This product has been referenced in:
  • Stradling JR  et al. Biomarkers of oxidative stress following continuous positive airway pressure withdrawal: data from two randomised trials. Eur Respir J 46:1065-71 (2015). Sandwich ELISA ; Human . Read more (PubMed: 26022961) »

See 1 Publication for this product

レビューと Q&A

Thank you for your call last week and for your patience while I have been in touch with the lab about your enquiry.

Theepitopse of the antibodies used in the kit have not been mapped at this point, and the kit has never been tested with sampl...

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Thank you for contacting us with your enquiry. I have looked through our catalogue and have found two sets that may be of interest to use. ab88404: http://www.abcam.com/Superoxide-Dismutase-1-antibody-ab88404.html and it's peptide ab99369: http...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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