Human stomach tissue lysate (signet-ring cell carcinoma) - insoluble fraction (male, 43 years) (ab44449)

製品の概要

  • 製品名Human stomach tissue lysate (signet-ring cell carcinoma) - insoluble fraction (male, 43 years)
    Stomach ライゼート 製品一覧
  • 製品の詳細
    Human stomach insoluble fraction
  • 特記事項

    Location: Lesser curvature. Diagnosis: Signet-ring cell carcinoma. Stage: II; T3N0M0. Grade: n/a Gross findings: Ulcerative lesion located at the lesser curvature, ill demarcated. Tumor measures 7 cm in diameter. Cut section is firm and white. Paragastric lymph nodes: 6. Microscopic findings: Tissue sections show proliferation of ring cells. Mitotic figures are evident. The neoplastic cells have coarse, irregular nuclei and scanty cytoplasm.The tumor cells form groups and glands and invade the musclaris propria, subserosa, and serosa. Lymph nodes show 0 of 7 positive.

    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab44449: Extraction 1: soluble protein fraction ab44447 Human Stomach Tumor Tissue lysate ab44446 Human Stomach Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab44449 Human Lung Tumor Tissue lysate ab44448 Human Lung Normal Tissue lysate (matched).

製品の特性

  • 製品の状態Liquid
  • 保存方法Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーpH: 7.40
    Constituents: PBS, 15.22% Thiourea, 30.03% Urea, 0.1% SDS, 0.77% DTT
  • Concentration information loading...
  • 特記事項(精製)Tissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
  • 研究分野

    Human stomach tissue lysate (signet-ring cell carcinoma) - insoluble fraction (male, 43 years) (ab44449) 使用論文

    ab44449 has not yet been referenced specifically in any publications.

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