Human stomach tissue lysate (adenocarcinoma) - insoluble fraction (male, 52 years) (ab44270)

製品の概要

  • 製品名Human stomach tissue lysate (adenocarcinoma) - insoluble fraction (male, 52 years)
    Stomach ライゼート 製品一覧
  • 製品の詳細
    Human stomach insoluble fraction
  • 特記事項

    Tissue: Stomach Location: Antrum. Diagnosis: Adenocarcinoma, poorly differentiated. Stage: II T3N0M0. Grade: n/a Sex: Male Age: 52 years Gross findings: Ulcerative lesion located at the antrum, ill demarcated. Ulceration measures 4 cm in diameter. Cut section is firm and white. Microscopic findings: Tissue sections show proliferation of malignant cells. The epithelial cells have small, basophilic, irregular nuclei and abundant cytoplasm, Cytoplasm contains much mucin. Nuclear chromatin is coarse with prominent nuclei. Mitotic figures are not evident. The neoplastic cells form groups and lines invading the smooth muscle propria and serosa. The stroma is infiltrated by small numbers of lymphocytes, plasma cells, eosinophils and neutrophils. Blood vessel invasions are not revealed. Lymph nodes show hyperplasia. Insoluble protein fraction.

     

    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab44270: Extraction 1: soluble protein fraction ab44268 Human Stomach Tumor Tissue lysate ab44267 Human Stomach Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab44270 Human Stomach Tumor Tissue lysate ab44269 Human Stomach Normal Tissue lysate (matched)

製品の特性

  • 製品の状態Liquid
  • 保存方法Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーpH: 7.40
    Constituents: PBS, 15.22% Thiourea, 30.03% Urea, 0.1% SDS, 0.77% DTT
  • Concentration information loading...
  • 特記事項(精製)Tissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
  • 研究分野

    Human stomach tissue lysate (adenocarcinoma) - insoluble fraction (male, 52 years) (ab44270) 使用論文

    ab44270 has not yet been referenced specifically in any publications.

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