Human stomach tissue lysate (adenocarcinoma) - insoluble fraction (female, 54 years) (ab44377)

製品の概要

  • 製品名Human stomach tissue lysate (adenocarcinoma) - insoluble fraction (female, 54 years)
    Stomach ライゼート 製品一覧
  • 製品の詳細
    Human stomach insoluble fraction
  • 特記事項

    Tissue: Stomach Location: Lesser curvature Diagnosis: Adenocarcinoma, poorly differentiated Stage: n/a Grade: n/a Gross findings: Hard thick ulceration located at the lesser curvature. Tumor size 3cm x 3cm in diameter. Cut section is soft, light yellow. Microscopic findings: Biopsy shows malignant columnar cells arranged in glands. The malignant cells have clear cytoplasm with vacuoles. Nuclei have irregular nuclear membranes, cytoplasm is granular or vacuolated with large nucleoli. Many monstrous nuclei. Many lymphocytes and the malignant cells have invaded the gastric wall.

    This product belongs to a range of individual sets of lysates that were extracted from very specific tissues and are gender-, cancer subtype-, tumor grade- and cellular morphology- specific. They allow for consistency, reproducibility, and comparative studies from experiment to experiment, due to the existence of specific (matched) lysates that can be used as controls. Two extractions from each tissue were performed, extraction 1 yielding the soluble protein fraction and extraction 2 the insoluble protein fraction. Please find below a range of products that relate to ab44377: Extraction 1: soluble protein fraction ab44375 Human Stomach Tumor Tissue lysate ab44374 Human Stomach Normal Tissue lysate (matched) Extraction 2: insoluble protein fraction ab44377 Human Stomach Tumor Tissue lysate ab44376 Human Stomach Normal Tissue lysate (matched)

製品の特性

  • 製品の状態Liquid
  • 保存方法Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーpH: 7.40
    Constituents: PBS, 15.22% Thiourea, 30.03% Urea, 0.1% SDS, 0.77% DTT
  • Concentration information loading...
  • 特記事項(精製)Tissue specimens are homogenized in modified RIPA buffer to obtain the soluble proteins, and centrifuged to clarify. The pellet was further extracted with a second buffer to obtain the less soluble protein fraction. The lysate solution may appear turbid at cold temperatures due to insolubility of buffer components. The solution should clear upon warming to room temperature.
  • 研究分野

    Human stomach tissue lysate (adenocarcinoma) - insoluble fraction (female, 54 years) (ab44377) 使用論文

    ab44377 has not yet been referenced specifically in any publications.

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