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Abcam’s Stem Cell Factor (Kit Ligand) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Stem Cell Factor concentrations in Human plasma, serum and cell culture supernatants.
A Stem Cell Factor specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Stem Cell Factor specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Stem Cell Factor captured in plate.
Store kit at 4°C immediately upon receipt, apart from the Streptavidin-Peroxidase Conjugate & Biotinylated Antibody, which should be stored at -20°C.
|内容||1 x 96 tests|
|100X Streptavidin-Peroxidase Conjugate||1 x 80µl|
|10X Diluent M Concentrate||1 x 20ml|
|20X Wash Buffer Concentrate||2 x 30ml|
|80X Biotinylated Human Stem Cell Factor Antibody||1 x 75µl|
|Chromogen Substrate||1 x 8ml|
|Sealing Tapes||3 units|
|Stem Cell Factor Microplate (12 x 8 well strips)||1 unit|
|Stem Cell Factor Standard||1 vial|
|Stop Solution||1 x 12ml|
Our Abpromise guarantee covers the use of ab108901 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent dilution.|