Human skin tissue lysate - total protein (ab30166)

製品の概要

  • 製品名Human skin tissue lysate - total protein
    Skin ライゼート 製品一覧
  • 製品の詳細
    Human skin total protein lysate
  • 特記事項


    Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot. Ethics: Human tissue samples are collected with the informed consent of the donors & relatives and are excised by licensed medical doctors. Tumor tissue samples are diagnosed and identified by at least two different evaluators. If you have more questions then please feel free to contact us. Donor information can be supplied on request.

  • アプリケーション適用あり: WBmore details

法規制情報

製品の特性

  • 製品の状態Liquid
  • 保存方法Store at -80°C. Allow to warm to room temp and agitate gently before aliquotting.
  • バッファーPreservative: None
    Constituents: 2% SDS, 10% Glycerol, 0.1% Bromophenol blue, Protease inhibitor, Sodium chloride, EDTA, Magnesium chloride, HEPES, 0.15M Beta mercaptoethanol
  • Concentration information loading...
  • ライゼート 備考Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot. Ethics: Human tissue samples are collected with the informed consent of the donors & relatives and are excised by licensed medical doctors. Tumor tissue samples are diagnosed and identified by at least two different evaluators. If you have more questions then please feel free to contact us. Donor information can be supplied on request.
  • 研究分野

    アプリケーション

    Our Abpromise guarantee covers the use of ab30166 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    WB Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.

    Human skin tissue lysate - total protein 画像

    • Anti-14-3-3 sigma antibody (ab94490) at 1 µg/ml + Human skin tissue lysate - total protein (ab30166) at 10 µg/ml

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Exposure time : 3 minutes

    Human skin tissue lysate - total protein (ab30166) 使用論文

    ab30166 has not yet been referenced specifically in any publications.

    Product Wall


    I checked with our laboratory and shipping department, and both confirmed that ab30166 is not shipped on dry ice while ab30158 is. The respective lysates have been found to be stable under their respective shipping conditions, so both lysates shou...

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    Thank you for your enquiry. The answer to your questions are as below. 1) The ages of the skin donors range from 21-86 years old. 2) We have skin tissues taken from skin from armpit, breast, buttocks (unexposed), buttocks upper back (exposed...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"