製品の概要

  • 製品名Human SIGLEC5 ELISA Kit
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 8 pg/ml
  • 検出範囲
    6.86 pg/ml - 5000 pg/ml
  • 添加回収試験

    100 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 101.3 91% - 108%
    Serum 106.3 93% - 114%
    Plasma 102.4 91% - 111%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s SIGLEC5 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human SIGLEC5 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human SIGLEC5 coated on a 96-well plate. Standards and samples are pipetted into the wells and SIGLEC5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human SIGLEC5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SIGLEC5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 関連性Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha2,3-linked and alpha2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. This molecule is expressed by monocytic/myeloid lineage cells. Found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus. Expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differenciation.
  • 別名
    • CD170
    • CD170 antigen
    • CD33 antigen like 2
    • CD33L2
    • mSiglec F
    • OB binding protein 2
    • OB BP2
    • OBBP2
    • Obesity binding protein 2
    • OTTMUSP00000041735
    • OTTMUSP00000041736
    • Sialic acid binding Ig like lectin 5
    • Sialic acid binding immunoglobulin like lectin 5
    • SIGLEC 5
    • Siglec F
    • Siglec F variant
    • Siglecf
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human SIGLEC5 ELISA Kit 画像

  • Representative standard curve using ab100641

  • Representative standard curve using ab100641

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Human SIGLEC5 ELISA Kit (ab100641) 使用論文

ab100641 has not yet been referenced specifically in any publications.

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