製品の概要

  • 製品名Human SDF1 alpha ELISA Kit
    SDF1 alpha キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall < 10%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall < 12%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 80 pg/ml
  • 検出範囲
    93.8 pg/ml - 6000 pg/ml
  • 添加回収試験

    > 80 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 94.63 86% - 104%
    Plasma 89.34 85% - 104%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s SDF1 alpha Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human SDF1 alpha in plasma and cell culture supernatants. (Human SDF1 alpha concentration is pretty low in normal plasma, it may not be detected in this assay). Serum samples are not recommended for use in this assay.

    This assay employs an antibody specific for Human SDF1 alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and SDF1 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human SDF1 alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SDF1 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples.

     

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 関連性SDF1 alpha and 1 beta are small cytokines that belong to the intercrine family, members of which activate leukocytes and are often induced by proinflammatory stimuli such as lipopolysaccharide, TNF or IL1. SDF1 alpha has been shown to be chemotactic for lymphocytes and is recently reported to be a ligand for CXCR4 (LESTR/fusin), a co-receptor for HIV1 entry into T cells. SDF-1 binding to CXCR4 inhibits HIV1 entry.
  • 別名
    • C-X-C motif chemokine 12
    • Chemokine (C X C motif) ligand 12
    • CXCL12
    • hIRH
    • Intercrine reduced in hepatomas
    • IRH
    • PBSF
    • Pre B cell growth stimulating factor
    • SCYB12
    • SDF 1
    • SDF 1 beta
    • SDF 1b
    • SDF1
    • SDF1 alpha (3-67)
    • SDF1 beta (3-72)
    • SDF1A
    • SDF1B
    • Stromal cell derived factor 1
    • TLSF a
    • TLSF b
    • TLSFa
    • TLSFb
    • TPAR1
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human SDF1 alpha ELISA Kit 画像

  • Representative standard curve using ab100637

  • Representative standard curve using ab100637

プロトコール

Human SDF1 alpha ELISA Kit (ab100637) 使用論文

This product has been referenced in:
  • Miles B  et al. Secondary lymphoid organ homing phenotype of human myeloid dendritic cells disrupted by an intracellular oral pathogen. Infect Immun 82:101-11 (2014). Sandwich ELISA ; Human . Read more (PubMed: 24126519) »

See 1 Publication for this product

Product Wall

The ELISA kit for SDF-1 alpha (aa 3-67) will detect the full length pro-SDF-1 (aa 1-93) and SDF-1 beta isoform (aa 3-72 ), albeit with a reduced affinity (approximately 25%). This has been tested empirically.

SDF1-CXCL12 human macrophage secretion

Excellent Excellent 5/5 (Ease of Use)
Abreviews
SDF1-CXCL12 secretion by human macrophages isolated by negative selection in culture for 3 or 7 days.
Kit easy to use, the assay takes about 5.30hr,
Secretion of CXCL12 by all different macrophage subtypes: M0(MCSF), M1(MSCF+IFNg), M2a (MSCF+IL4), M2c-DEX (MCSF+Dexamethasone), M2c-IL10(MCSF+IL10), M2c-TGFb(MCSF+TGFb)
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Abcam user community

Verified customer

投稿 Apr 01 2014

If the background is already low though, there are a few options to increase the overall OD responses from your samples. The optional overnight standard/sample incubation is the easiest way but since you have already tried this some other methods are b...

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I am happy to confirm that any of the 3 anti-coagulants should be fine to be used for testing plasma samples with the SDF1 alpha Human ELISA Kit (ab100637).

Merci de votre émail.

Nous allons donc ajouter un kit entier à votre prochaine commande. Est-ce que vous pouvez s'il vous plaît nous renvoyer le tube de HRP-Streptavidin?

Merci beaucoup.

Merci pour votre émail.

Je peux vous confirmer que le produit doit arriver demain chez vous. Le numéro de commande est le xx.

Je suis désolée du délais et des inconvenances. Veuillez ne pas h&eacut...

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Merci de votre confirmation.

Nous sommes en train de procéder au remplacement et il va être ajouté à une de vos prochaines commandes. Nous allons vous confirmer laquelle dès que nous pouvons.

Merci ...

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Merci de nous avoir contacté. Nous sommes en train d'organiser un remplacement. Est-ce que vous pouvez nous confirmer que le tube était bien centrifugé aussi pour vérifier que le liquid n'étais pas dans le capuchon? <...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one kit of ab100637.
To check the status of the order please contact our Cus...

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Congratulations on your new arrival!
I will send out that replacement kit of ab100637. As you are out of the lab, to whose attention should I send shipments to?

1-10 of 21 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"