製品の概要

  • 製品名
    Human SCF ELISA Kit
    SCF キット 製品一覧
  • 検出方法
    Colorimetric
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 2 pg/ml
  • 検出範囲
    2.74 pg/ml - 2000 pg/ml
  • 添加回収試験

    97 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 101.3 85% - 106%
    Serum 94.29 80% - 103%
    Plasma 95.48 82% - 104%

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Rat, Human
  • 製品の概要

    Abcam’s SCF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human SCF in serum, plasma and cell culture supernatants.


    This assay employs an antibody specific for Human SCF coated on a 96-well plate. Standards and samples are pipetted into the wells and SCF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human SCF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SCF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get higher sensitivity in only 90 minutes with Human Stem Cell Factor ELISA Kit (ab176109) from our SimpleStep ELISA® range


     

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

  • 保存方法
    Store at -20°C. Please refer to protocols.
  • 内容 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    400X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human SCF (lyophilized) 2 vials
    Recombinant Human SCF Standard 2 vials
    SCF Microplate (12 x 8 wells) 1 unit
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • 研究分野
  • 機能
    Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
  • 関連疾患
    Hyperpigmentation with or without hypopigmentation, familial progressive
    Deafness, congenital, unilateral or asymmetric
  • 配列類似性
    Belongs to the SCF family.
  • 発生段階
    Acts in the early stages of hematopoiesis.
  • 翻訳後修飾
    A soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain.
    Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97.
    A soluble form exists as a cleavage product of the extracellular domain.
  • 細胞内局在
    Secreted; Cell membrane and Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane. Cell projection, lamellipodium. Cell projection, filopodium.
  • Information by UniProt
  • 別名
    • C kit ligand
    • C-kit ligand
    • Ckit ligand
    • DCUA
    • DFNA69
    • DKFZp686F2250
    • familial progressive hyperpigmentation 2
    • FPH2
    • FPHH
    • KIT ligand
    • Kitl
    • KITLG
    • KL 1
    • KL1
    • Mast cell growth factor
    • MGF
    • MGF stem cell factor
    • SCF
    • SCF_HUMAN
    • SF
    • SHEP7
    • sKITLG
    • Soluble KIT ligand
    • Steel factor
    • steel, mouse, homolog of
    • Stem cell factor
    • Stem cell factor precursor
    see all
  • 参照データベース

    関連製品

    アプリケーション

    Our Abpromise guarantee covers the use of ab100636 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Sandwich ELISA Use at an assay dependent concentration.

    画像

    • ELISA: ab100636 SCF Human ELISA Kit
      ELISA: ab100636 SCF Human ELISA Kit

      Human SCF measured in undiluted cell culture supernatants (duplicates +/- SD).

    • ELISA: ab100636 SCF Human ELISA Kit
      ELISA: ab100636 SCF Human ELISA Kit

      Human SCF measured in undiluted human biological fluids (duplicates +/- SD). Mouse and rat sera and plasma showed undetectable levels.

    • ELISA: ab100636 SCF Human ELISA Kit
      ELISA: ab100636 SCF Human ELISA Kit

      Standard curves in different buffers with background signal subtracted (duplicates; +/- SD).

    • Typical standard curve
      Typical standard curve

      Representative standard curve using ab100636

    • Typical standard curve
      Typical standard curve

      Representative standard curve using ab100636

    プロトコール

    参考文献

    This product has been referenced in:
    • Feng J  et al. Role of stem cell factor in the regulation of ICC proliferation and detrusor contraction in rats with an underactive bladder. Mol Med Rep 16:1516-1522 (2017). Rat . Read more (PubMed: 28627603) »

    See 1 Publication for this product

    ab100636 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    3 reviews or Q&A

    10
    Votes: Highest First

    Allelic Mutations of KITLG, Encoding KIT Ligand, Cause Asymmetric and Unilateral Hearing Loss and Waardenburg Syndrome Type 2.

     Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Sample: Cell-free supernatants from NIH 3T3 cell culture centrifuged at 15,000 rpm for 10 min at 4ºC. Samples were stored at -70ºC until the ELISAs were performed.
    Amount of Sample: 50 ul
    Type of Kit Used: ELISA
    Manufacturer instructions were followed.
    Additional data
    We diluted 50 ul of supernatants in 50 ul of 1XAssay Diluent B
    Washings were done on a Microplate Washer machine with 300 ul of wash buffer.
    The standards data generated an excellent curve.
    PubMed ID: 26522471
    abreview image
    Username

    Abcam user community

    Verified customer

    投稿 Nov 21 2015

    The sample tested were supernatants (IMDM+30%FBS and StemSpan serum free medium) which have been in contact with Mononuclear cells.

    - I didn’t dilute the samples

    - The only step altered was the overnight incubation at 4C (step 2...

    Read More

    Thank you very much for sending the details. These were very helpful.

    As we discussed on phone please try the experiment again by keeping all reagents at room temperature.

    Please let me know if you need further assistance.

    ELISA Kit: Why there are 2 vilas of secondary antibody?

    Thank you very much for your phone call this morning.

    As discussed I will be followingthis case further. Could you please provide the following details;

    - Types of sample used. If supernatant, what type of cells were incubated
    - D...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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