97
%
サンプルの種類 | 平均 % | 測定範囲 |
---|---|---|
Cell culture supernatant | 101.3 | 85% - 106% |
Serum | 94.29 | 80% - 103% |
Plasma | 95.48 | 82% - 104% |
Abcam’s SCF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human SCF in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human SCF coated on a 96-well plate. Standards and samples are pipetted into the wells and SCF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human SCF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SCF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get higher sensitivity in only 90 minutes with Human Stem Cell Factor ELISA Kit (ab176109) from our SimpleStep ELISA® range
Optimization may be required with urine samples.
内容 | 1 x 96 tests |
---|---|
20X Wash Buffer Concentrate | 1 x 25ml |
400X HRP-Streptavidin Concentrate | 1 x 200µl |
5X Assay Diluent B | 1 x 15ml |
Assay Diluent A | 1 x 30ml |
Biotinylated anti-Human SCF (lyophilized) | 2 vials |
Recombinant Human SCF Standard | 2 vials |
SCF Microplate (12 x 8 wells) | 1 unit |
Stop Solution | 1 x 8ml |
TMB One-Step Substrate Reagent | 1 x 12ml |
Our Abpromise guarantee covers the use of ab100636 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Sandwich ELISA | Use at an assay dependent concentration. |
Human SCF measured in undiluted cell culture supernatants (duplicates +/- SD).
Human SCF measured in undiluted human biological fluids (duplicates +/- SD). Mouse and rat sera and plasma showed undetectable levels.
Standard curves in different buffers with background signal subtracted (duplicates; +/- SD).
Representative standard curve using ab100636
Representative standard curve using ab100636
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"