製品の概要

  • 製品名Human SCF ELISA Kit
    SCF キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 2 pg/ml
  • 検出範囲
    2.74 pg/ml - 2000 pg/ml
  • 添加回収試験

    97 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 101.3 85% - 106%
    Serum 94.29 80% - 103%
    Plasma 95.48 82% - 104%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s SCF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human SCF in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human SCF coated on a 96-well plate. Standards and samples are pipetted into the wells and SCF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human SCF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of SCF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 関連性Stem Cell Factor (SCF) is a hematopoietic growth factor that exerts its activity at the early stages of hematopoiesis. SCF stimulates the proliferation of mast cells and is able to augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in bone marrow culture. It also mediates cell to cell adhesion and acts synergistically with other cytokines, probably interleukins. A soluble form is produced by proteolytic processing of isoform 1 in the extracellular domain.
  • 別名
    • C kit ligand
    • Ckit ligand
    • Kit ligand
    • Kitl
    • KITLG
    • KL 1
    • KL1
    • Mast cell growth factor
    • MGF
    • SF
    • SHEP7
    • Steel factor
    • Stem cell factor
    • Stem cell factor precursor
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100636 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human SCF ELISA Kit 画像

  • Human SCF measured in undiluted cell culture supernatants (duplicates +/- SD).

  • Human SCF measured in undiluted human biological fluids (duplicates +/- SD). Mouse and rat sera and plasma showed undetectable levels.

  • Standard curves in different buffers with background signal subtracted (duplicates; +/- SD).

  • Representative standard curve using ab100636

  • Representative standard curve using ab100636

プロトコール

Human SCF ELISA Kit (ab100636) 使用論文

ab100636 has not yet been referenced specifically in any publications.

Product Wall

Sample: Cell-free supernatants from NIH 3T3 cell culture centrifuged at 15,000 rpm for 10 min at 4ºC. Samples were stored at -70ºC until the ELISAs were performed.
Amount of Sample: 50 ul
Type of Kit Used: ELISA
Manufacturer instructions were followed.
Additional data
We diluted 50 ul of supernatants in 50 ul of 1XAssay Diluent B
Washings were done on a Microplate Washer machine with 300 ul of wash buffer.
The standards data generated an excellent curve.
PubMed ID: 26522471
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投稿 Nov 21 2015

Thank you very much for sending the details. These were very helpful.

As we discussed on phone please try the experiment again by keeping all reagents at room temperature.

Please let me know if you need further assistance.

Thank you very much for your phone call this morning.

As discussed I will be followingthis case further. Could you please provide the following details;

- Types of sample used. If supernatant, what type of cells were incubated
- D...

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