製品の概要

  • 製品名Human RPE65 peptide

製品の詳細

  • 由来Synthetic
  • アミノ酸配列
    • アクセッション番号Q16518
    • 生物種Human

関連製品

特性

Our Abpromise guarantee covers the use of ab99880 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Blocking - Blocking peptide for Anti-RPE65 antibody (ab67042)

  • 精製度70 - 90% by HPLC.

  • 製品の状態Liquid
  • 備考

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

関連情報

  • 別名
    • All-trans-retinyl-palmitate hydrolase
    • LCA 2
    • LCA2
    • Leber congenital amaurosis
    • mRPE 65
    • mRPE65
    • p63
    • rd 12
    • rd12
    • Retinal pigment epithelium specific 61 kDa protein
    • Retinal pigment epithelium specific 65 kDa protein
    • Retinal pigment epithelium specific protein
    • Retinal pigment epithelium specific protein 65kDa
    • Retinal pigment epithelium-specific 65 kDa protein
    • Retinitis pigmentosa 20
    • Retinoid isomerohydrolase
    • Retinol isomerase
    • RP 20
    • RP20
    • RPE 65
    • RPE65
    • RPE65_HUMAN
    • sRPE 65
    • sRPE65
    see all
  • 機能Plays important roles in the production of 11-cis retinal and in visual pigment regeneration. The soluble form binds vitamin A (all-trans-retinol), making it available for LRAT processing to all-trans-retinyl ester. The membrane form, palmitoylated by LRAT, binds all-trans-retinyl esters, making them available for IMH (isomerohydrolase) processing to all-cis-retinol. The soluble form is regenerated by transferring its palmitoyl groups onto 11-cis-retinol, a reaction catalyzed by LRAT. The enzymatic activity is linearly dependent of the expression levels and membrane association.
  • 組織特異性Retinal pigment epithelium specific.
  • 関連疾患Defects in RPE65 are the cause of Leber congenital amaurosis type 2 (LCA2) [MIM:204100]. LCA designates a clinically and genetically heterogeneous group of childhood retinal degenerations, generally inherited in an autosomal recessive manner. Affected infants have little or no retinal photoreceptor function as tested by electroretinography. LCA represents the most common genetic cause of congenital visual impairment in infants and children.
    Defects in RPE65 are the cause of retinitis pigmentosa type 20 (RP20) [MIM:613794]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP20 inheritance is autosomal dominant.
  • 配列類似性Belongs to the carotenoid oxygenase family.
  • 翻訳後修飾Palmitoylation by LRAT regulates ligand binding specificity; the palmitoylated form (membrane form) specifically binds all-trans-retinyl-palmitate, while the soluble unpalmitoylated form binds all-trans-retinol (vitamin A).
  • 細胞内局在Cytoplasm. Cell membrane. Attached to the membrane by a lipid anchor when palmitoylated (membrane form), soluble when unpalmitoylated.
  • Information by UniProt

Human RPE65 peptide (ab99880) 使用論文

ab99880 has not yet been referenced specifically in any publications.

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