Human Anti-Respiratory syncytial virus IgM ELISA Kit (RSV) (ab108766)
Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
製品の概要
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製品名
Human Anti-Respiratory syncytial virus IgM ELISA Kit (RSV) -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Pos.Serum 24 2.6% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Pos.Serum 12 5.4% -
サンプルの種類
Serum, Hep Plasma, Cit plasma -
アッセイタイプ
Indirect -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s anti-Respiratory syncytial virus (RSV) IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Respiratory syncytial virus in Human serum and plasma.
A 96-well plate has been precoated with Respiratory syncytial virus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Respiratory syncytial virus -specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Respiratory syncytial virus IgM sample captured in plate.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 ラベル 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Anti-Respiratory syncytial virus (IgM) Coated Microplate (12 x 8 wells) 1 unit IgM Sample Diluent Colored Green; White cap 1 x 100ml Respiratory syncytial virus anti-IgM HRP Conjugate Colored Red; Black cap 1 x 20ml Respiratory syncytial virus IgM Cut-off Control Colored yellow; Green cap 1 x 3ml Respiratory syncytial virus IgM Negative Control Colored yellow; Blue cap 1 x 2ml Respiratory syncytial virus IgM Positive Control Colored yellow; Red cap 1 x 2ml Stop Solution red cap 1 x 15ml TMB Substrate Solution Yellow cap 1 x 15ml -
研究分野
画像
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Specific antigens are coated on the 96-well plate, controls or test samples are added to the well and incubated. The wells are washed to remove any unbound Human anti-antigen antibodies (Ig). A horseradish peroxidase (HRP) labelled anti-Human Ig conjugate is added to the wells. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The intensity of yellow coloration is directly proportional to the amount of Human anti-antigen Ig captured on the plate.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab108766 は 1 報の論文で使用されています。
- Mandi H et al. Seroepidemiology of respiratory syncytial virus infection in rural and semi-rural areas of the Littoral region of Cameroon. BMC Infect Dis 21:144 (2021). PubMed: 33541281