Human Resistin ELISA Kit (RETN) (ab108896)
Key features and details
- Sensitivity: 0.2 ng/ml
- Range: 0.25 ng/ml - 16 ng/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human Resistin ELISA Kit (RETN)
Resistin キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall 5.3% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall 10.2% -
サンプルの種類
Cell culture supernatant, Urine, Serum, Plasma, Cerebral Spinal Fluid -
アッセイタイプ
Sandwich (quantitative) -
検出感度
= 0.2 ng/ml -
検出範囲
0.25 ng/ml - 16 ng/ml -
添加回収試験
= 97 %
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全工程の試験時間
5h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Resistin (RETN) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Resistin concentrations in Human plasma, serum, urine, saliva and cell culture supernatants.
A Resistin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Resistin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Resistin captured in plate.
Get results in 90 minutes with Human Resistin ELISA Kit (ab183364) from our SimpleStep ELISA® range.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Resistin Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Resistin Microplate (12 x 8 well strips) 1 unit Resistin Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究分野
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機能
Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes. -
組織特異性
Expressed only in fatty tissues. -
配列類似性
Belongs to the resistin/FIZZ family. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Adipose tissue specific secretory factor
- Adipose tissue-specific secretory factor
- ADSF
see all -
参照データベース
- Entrez Gene: 56729 Human
- Omim: 605565 Human
- SwissProt: Q9HD89 Human
- Unigene: 283091 Human
関連製品
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SimpleStep ELISA kits
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab108896 は 2 報の論文で使用されています。
- Lillyman DJ et al. Axial hypersensitivity is associated with aberrant nerve sprouting in a novel model of disc degeneration in female Sprague Dawley rats. JOR Spine 5:e1212 (2022). PubMed: 36203864
- Al-Rawi N & Al-Marzooq F The Relation between Periodontopathogenic Bacterial Levels and Resistin in the Saliva of Obese Type 2 Diabetic Patients. J Diabetes Res 2017:2643079 (2017). PubMed: 29138754