Human Resistin ELISA Kit (RETN) (ab108896)
製品の概要
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製品名Human Resistin ELISA Kit (RETN)
Resistin キット 製品一覧 -
検出方法Colorimetric
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再現性
Intra-Assay（同時再現性） サンプル N 平均値 SD CV% Overall 4.6% Inter-Assay（日差再現性） サンプル N 平均値 SD CV% Overall 7% -
サンプルの種類Cell culture supernatant, Urine, Serum, Plasma
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アッセイタイプSandwich (quantitative)
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検出感度= 0.2 ng/ml
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検出範囲0.25 ng/ml - 16 ng/ml
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添加回収試験
= 97 %
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全工程の試験時間5h 00m
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ステップMultiple steps standard assay
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種交差性交差種: Human
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製品の概要
Abcam’s Resistin (RETN) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Resistin concentrations in Human plasma, serum, urine, saliva and cell culture supernatants.
A Resistin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Resistin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Resistin captured in plate.
Get results in 90 minutes with Human Resistin ELISA Kit (ab183364) from our SimpleStep ELISA® range.
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アプリケーション適用あり: Sandwich ELISAmore details
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試験プラットフォームMicroplate
製品の特性
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保存方法Store at -20°C. Please refer to protocols.
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内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Resistin Antibody 1 x 120µl Chromogen Substrate 1 x 8ml Resistin Microplate (12 x 8 well strips) 1 unit Resistin Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml -
研究分野
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機能Hormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes.
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組織特異性Expressed only in fatty tissues.
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配列類似性Belongs to the resistin/FIZZ family.
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細胞内局在Secreted.
- Information by UniProt
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別名
- Adipose tissue specific secretory factor
- Adipose tissue-specific secretory factor
- ADSF
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参照データベース
- Entrez Gene: 56729 Human
- Omim: 605565 Human
- SwissProt: Q9HD89 Human
- Unigene: 283091 Human
関連製品
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SimpleStep ELISA kits
アプリケーション
Our Abpromise guarantee covers the use of ab108896 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent dilution. |
画像
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Typical Standard CurveRepresentative Standard Curve using ab108896.
プロトコール
参考文献
This product has been referenced in:
- Al-Rawi N & Al-Marzooq F The Relation between Periodontopathogenic Bacterial Levels and Resistin in the Saliva of Obese Type 2 Diabetic Patients. J Diabetes Res 2017:2643079 (2017). Read more (PubMed: 29138754) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"