The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Blocking - Blocking peptide for Anti-PTP lambda antibody (ab13223)
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions. - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer. - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent. - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised. - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
Pancreatic carcinoma phosphatase 2
Protein-tyrosine phosphatase J
Receptor-type tyrosine-protein phosphatase U
PTP lambda is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle and oncogenic transformation. PTP lambda is a large protein that possesses a C-terminus PTP domain and multiple noncatalytic domains which include a domain with similarity to band 4.1 superfamily of cytoskeletal-associated proteins, a region consisting of five PDZ domains and a leucine zipper motif. Four alternatively spliced transcript variants, which encode distinct proteins, have been reported.
PTP lambda is up-regulated upon cell contact and is found in high levels in brain, pancreas, and skeletal muscle; less in colon, kidney, liver, stomach and uterus. It is not expressed in placenta and spleen.
Type 1 membrane protein
PTP lambda Western Blot (ab13230)
All lanes : Anti-PTP lambda antibody (ab13223) at 1/500 dilution
Lane 1 : Human brain lysate Lane 2 : Human kidney lysate Lane 3 : Human pancreas lysate Lane 4 : Human heart lysate Lane 5 : Human brain lysate with Human PTP lambda peptide (ab13230) at 1 µg/ml Lane 6 : Human kidney lysate with Human PTP lambda peptide (ab13230) at 1 µg/ml Lane 7 : Human pancreas lysate with Human PTP lambda peptide (ab13230) at 1 µg/ml Lane 8 : Human heart lysate with Human PTP lambda peptide (ab13230) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/5000 dilution
Performed under reducing conditions.
Exposure time: 5 minutes
A weak band of predicted size (~160kDa) is observed in human kidney lysate which is quenched by the blocking peptide (ab13230).
has not yet been referenced specifically in any publications.