Human Protein S ELISA Kit (ab125969)
Key features and details
- Sensitivity: 0.22 µg/ml
- Range: 0.25 µg/ml - 8 µg/ml
- Sample type: Cell culture supernatant, Cell Lysate, Plasma, Serum, Tissue
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
製品の概要
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製品名
Human Protein S ELISA Kit
Protein S キット 製品一覧 -
検出方法
Colorimetric -
再現性
Intra-Assay(同時再現性) サンプル N 平均値 SD CV% Overall 5.9% Inter-Assay(日差再現性) サンプル N 平均値 SD CV% Overall 10.4% -
サンプルの種類
Cell culture supernatant, Serum, Plasma, Tissue, Cell Lysate -
アッセイタイプ
Competitive -
検出感度
0.22 µg/ml -
検出範囲
0.25 µg/ml - 8 µg/ml -
添加回収試験
97 %
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全工程の試験時間
4h 00m -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s Protein S Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human protein S in plasma, serum, cell culture supernatants cell lysate and tissue samples.
A Protein S specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Protein S is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Protein S captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 1X Biotinylated Human Protein S (Lyophilized) 1 vial 20X Wash Buffer Concentrate 1 x 30ml Chromogen Substrate 1 x 7ml Protein S Microplate (12 x 8 well strips) 1 unit Protein S Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
研究分野
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機能
Anticoagulant plasma protein; it is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa. It helps to prevent coagulation and stimulating fibrinolysis. -
組織特異性
Plasma. -
関連疾患
Defects in PROS1 are the cause of protein S deficiency (PROS1D) [MIM:612336]; also known as thrombophilia due to protein S deficiency. PROS1D is a cause of hereditary thrombophilia, a hemostatic disorder characterized by impaired regulation of blood coagulation and a tendency to recurrent venous thrombosis. However, many adults with heterozygous disease may be asymptomatic. Based on the plasma levels of total and free PROS1 antigen as well as the serine protease-activated protein C cofactor activity, three types of PROS1D have been described: type I, characterized by reduced total and free PROS1 antigen levels together with reduced anticoagulant activity; type III, in which only free PROS1 antigen and PROS1 activity levels are reduced; and the rare type II which is characterized by normal concentrations of both total and free PROS1 antigen, but low cofactor activity. -
配列類似性
Contains 4 EGF-like domains.
Contains 1 Gla (gamma-carboxy-glutamate) domain.
Contains 2 laminin G-like domains. -
翻訳後修飾
The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains. -
細胞内局在
Secreted. - Information by UniProt
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別名
- Preproprotein S
- Propiece of latent protein S
- PROS
see all -
参照データベース
- Entrez Gene: 5627 Human
- Omim: 176880 Human
- SwissProt: P07225 Human
- Unigene: 64016 Human
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab125969 は 1 報の論文で使用されています。
- Liang Y et al. Coagulation cascade and complement system in systemic lupus erythematosus. Oncotarget 9:14862-14881 (2018). PubMed: 29599912