製品の概要

  • 製品名Human Prolactin ELISA Kit
    Prolactin キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Level 1 20 6.32 0.4 6.3%
    Level 2 20 19.22 1.19 6.18%
    Level 3 20 54.52 2.64 4.84%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Level 1 20 6.58 0.45 6.77%
    Level 2 20 21.34 1.3 6.11%
    Level 3 20 58.39 2.93 5.01%
  • テスト
    1 x 96 test
  • サンプルの種類
    Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    0.25 ng/ml
  • 検出範囲
    5 ng/ml - 100 ng/ml
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Prolactin in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Prolactin in Human serum.

    Microtiterstrip wells are precoated with inactivated specific Corynebacterium diphtheriae toxin (toxoid) antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-human IgG conjugate is added. This conjugate binds to the captured C. diphtheriae toxin-specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of C. diphtheriae toxin-specific IgG antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 関連性Various hormones are secreted from the anterior pituitary during development and growth. Prolactin is a growth factor secreted by the anterior pituitary that is necessary for the proliferation and differentiation of the mammary glands. Prolactin acts primarily on the mammary gland by promoting lactation.
  • 細胞内局在Secreted
  • 別名
    • AV290867
    • Lactogenic Hormone
    • Luteotropic Hormone
    • Mammotropin
    • OTTMUSP00000000651
    • PRL
    • Prl1a1
    • Prol
    • Prolactin precursor
    • RATPRLSD1
    • RNPROL
    • RP23-351I6.2
    • RP3-404K8.1
    see all
  • 参照データベース

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab108679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent dilution.

プロトコール

Human Prolactin ELISA Kit (ab108679) 使用論文

ab108679 has not yet been referenced specifically in any publications.

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Thank you for contacting Abcam regarding ab108679.


I have confirmed that both antibodies in this kit are derived from Mouse.


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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"