製品の概要

  • 製品名
    Human PMN Elastase ELISA Kit
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4.8%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 5.6%
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    1.98 pg/ml
  • 検出範囲
    0.16 ng/ml - 10 ng/ml
  • 添加回収試験

    104 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Plasma 104 96% - 110%

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s PMN Elastase Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human PMN Elastase concentrations in Cell culture supernatants, plasma, exudate, bronchoalveolar lavage fluid, cerebrospinal fluid and seminal plasma.


    PMN Elastase specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an HRP-conjugated PMN Elastase detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the PMN Elastase amount of sample captured in plate. 


    The capture antibody in the Human PMN-Elastase ELISA kit is specific to PMN elastase, while the detection antibody is specific to alpha1-PI, hence the kit is specific to the PMN Elastase/alpha1-PI complex.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab119553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • Standard curve with background signal subtracted (duplicates; +/- SD).

  • PMN elastase measured in the high control and low control (ab119553) and in biological fluids (dilution range 1/1-1/1000; duplicates +/- SD).

  • Representative Standard Curve using ab119553.

プロトコール

参考文献

This product has been referenced in:
  • Thålin C  et al. Citrullinated histone H3 as a novel prognostic blood marker in patients with advanced cancer. PLoS One 13:e0191231 (2018). ELISA ; Human . Read more (PubMed: 29324871) »
  • Schechter MC  et al. Neutrophil extracellular trap (NET) levels in human plasma are associated with active TB. PLoS One 12:e0182587 (2017). ELISA ; Human . Read more (PubMed: 28777804) »

See all 7 Publications for this product

レビューと Q&A

Filter by Ratings

Elastase levels in gut mucosa lysates

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Followed the kit's protocol from start to finish.
The samples were whole tissue lysates of human gut mucosa which were assayed in various dilutions (in PBS). Have not tried any other sample type.
Kit is very easy to use and works just like any standard ELISA.
Got consitently good results. Will buy it again.
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Verified customer

投稿 Mar 13 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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