製品の概要

  • 製品名Human PLGF ELISA Kit
    PLGF キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 2 pg/ml
  • 検出範囲
    1.372 pg/ml - 1000 pg/ml
  • 添加回収試験

    89 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 103.4 75% - 115%
    Serum 88.09 79% - 96%
    Plasma 75.69 68% - 93%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s PLGF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PLGF in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human PLGF coated on a 96-well plate. Standards and samples are pipetted into the wells and PLGF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human PLGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PGF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner.
  • 組織特異性While the three isoforms are present in most placental tissues, PlGF-2 is specific to early (8 week) placenta and only PlGF-1 is found in the colon and mammary carcinomas.
  • 配列類似性Belongs to the PDGF/VEGF growth factor family.
  • ドメインIsoform PlGF-2 contains a basic insert which acts as a cell retention signal.
  • 翻訳後修飾N-glycosylated.
  • 細胞内局在Secreted. The three isoforms are secreted but PlGF-2 appears to remain cell attached unless released by heparin.
  • Information by UniProt
  • 別名
    • AI854365
    • D12S1900
    • MGC93298
    • Pgf
    • PGFL
    • PIGF
    • Placenta growth factor
    • Placental growth factor
    • Placental growth factor vascular endothelial growth factor related protein
    • PlGF
    • PlGF 2
    • PLGF_HUMAN
    • PlGF2
    • SHGC 10760
    • Vascular endothelial growth factor related protein
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100629 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human PLGF ELISA Kit 画像

  • Representative Standard Curve using ab100629.

プロトコール

Human PLGF ELISA Kit (ab100629) 使用論文

ab100629 has not yet been referenced specifically in any publications.

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