Anti-Human placental lactogen (epitope 2) 抗体 [INN-hPL-37] (ab11396)

製品の概要

  • 製品名Anti-Human placental lactogen (epitope 2) antibody [INN-hPL-37]
  • 製品の詳細
    Mouse monoclonal [INN-hPL-37] to Human placental lactogen (epitope 2)
  • 特異性Radioimmunological direct binding studies were performed with 125I-labelled. hPL, hGH, hPRL and increasing amounts of INN-hPL-37. In addition, competitive RIA with unlabelled hGH, hPRL and hPL were used for characterization. In competitive RIA cross reactivity with hGH and hPRL was below 1%. Direct binding demonstrated 39% reactivity with hGH but no cross reactivity with hPRL.
  • アプリケーション適用あり: RIAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Human placental lactogen.

  • 特記事項This product should be stored undiluted. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide.
  • Concentration information loading...
  • 精製度Protein A purified
  • 特記事項(精製)Purification by affinity chromatography.
  • ポリ/モノモノクローナル
  • クローン名INN-hPL-37
  • ミエローマx63-Ag8.653
  • アイソタイプIgG1
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab11396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
RIA Use at an assay dependent dilution.

ターゲット情報

  • 関連性Placental lactogen is a member of the somatotropin/prolactin family of hormones and plays an important role in growth control. The gene is located at the growth hormone locus on chromosome 17 along with four other related genes in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. Although the five genes share a remarkably high degree of sequence identity, they are expressed selectively in different tissues. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed mainly in the placenta and utilizes multiple transcription initiation sites.
  • 細胞内局在Secreted
  • 参照データベース
  • 別名
    • choriomammotropin antibody
    • Chorionic somatomammotropin A antibody
    • Chorionic somatomammotropin hormone 1 (placental lactogen) antibody
    • chorionic somatomammotropin hormone 1 antibody
    • Chorionic somatomammotropin hormone 2 antibody
    • CS 1 antibody
    • CS-2 antibody
    • CS-B antibody
    • CSA antibody
    • CSB antibody
    • CSH1 antibody
    • CSMT antibody
    • hCSB antibody
    • Lactogen precursor antibody
    • Lactogen, placental antibody
    • PL antibody
    • placental lactogen antibody
    see all

Anti-Human placental lactogen (epitope 2) antibody [INN-hPL-37] 画像

  • ab11396 at 1/100 staining human BeWo cells (placenta, choriocarcinoma) by ICC/IF. The cells were paraformaldehyde fixed and blocked with BSA prior to incubation with the antibody for 30 minutes. A FITC conjugated goat anti-mouse IgG antibody was used as the secondary antibody. The image is shown in the upper right hand panel. In this experiment ab15554 was used as a positive control (bottom left hand panel). Cells were counter stained with DAPI (upper left hand panel).

    See Abreview

Anti-Human placental lactogen (epitope 2) antibody [INN-hPL-37] (ab11396) 使用論文

ab11396 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Recombinant protein (Human recombinant hPL)
Loading amount 0.1 µg
Specification Human recombinant hPL
Blocking step Milk as blocking agent for 17 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. Stoil Dmitrov

Verified customer

投稿 Apr 16 2007

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (BeWo, placenta, choriocarcinoma)
Specification BeWo, placenta, choriocarcinoma
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1%
Username

Dr. Stoil Dmitrov

Verified customer

投稿 Apr 11 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"