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|Cell culture supernatant||108.2||101% - 116%|
|Serum||122.4||110% - 130%|
|Plasma||119.6||111% - 127%|
Abcam’s PF4 (CXCL4) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PF4 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human PF4 coated on a 96-well plate. Standards and samples are pipetted into the wells and PF4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human PF4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PF4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get results in 90 minutes with Human PF4 ELISA Kit (CXCL4) (ab189573) from our SimpleStep ELISA® range.
Optimization may be required with urine samples.
|内容||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|500X HRP-Streptavidin||1 x 200µl|
|5X Assay Diluent B||1 x 15ml|
|Assay Diluent C||1 x 30ml|
|Biotinylated anti-Human PF4||2 units|
|PF4 Microplate (12 x 8 wells)||1 unit|
|Recombinant Human PF4 Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100628 in the following tested applications.
|Sandwich ELISA||Use at an assay dependent concentration.|
Human PF4 measured in biological fluids showing quantity (pg) per mL of tested sample. Samples were diluted 200-800 fold.
Representative Standard Curve using ab100628.
ab100628 has not yet been referenced specifically in any publications.