Human PF4 ELISA Kit (CXCL4) (ab100628)
Key features and details
- Sensitivity: 20 pg/ml
- Range: 20.58 pg/ml - 15000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
製品の概要
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製品名
Human PF4 ELISA Kit (CXCL4)
PF4 キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Cell culture supernatant, Serum, Plasma -
アッセイタイプ
Sandwich (quantitative) -
検出感度
< 20 pg/ml -
検出範囲
20.58 pg/ml - 15000 pg/ml -
添加回収試験
100 %
特定サンプルでの回収試験 サンプルの種類 平均 % 測定範囲 Cell culture supernatant 108.2 101% - 116% Serum 122.4 110% - 130% Plasma 119.6 111% - 127% -
ステップ
Multiple steps standard assay -
種交差性
交差種: Human -
製品の概要
Abcam’s PF4 (CXCL4) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PF4 in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human PF4 coated on a 96-well plate. Standards and samples are pipetted into the wells and PF4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human PF4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PF4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get results in 90 minutes with Human PF4 ELISA Kit (CXCL4) (ab189573) from our SimpleStep ELISA® range.
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特記事項
Optimization may be required with urine samples.
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試験プラットフォーム
Microplate
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 1 x 96 tests 20X Wash Buffer 1 x 25ml 500X HRP-Streptavidin 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated anti-Human PF4 2 units PF4 Microplate (12 x 8 wells) 1 unit Recombinant Human PF4 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
研究分野
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機能
Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation, the short form is a more potent inhibitor than the longer form. -
配列類似性
Belongs to the intercrine alpha (chemokine CxC) family. -
翻訳後修飾
Binds non-covalently to a proteoglycan molecule. -
細胞内局在
Secreted. - Information by UniProt
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別名
- C-X-C motif chemokine 4
- Chemokine (C X C motif) ligand 4
- Chemokine (CXC motif) ligand 4
see all -
参照データベース
- Entrez Gene: 5196 Human
- Omim: 173460 Human
- SwissProt: P02776 Human
- Unigene: 81564 Human
関連製品
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SimpleStep ELISA kits
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab100628 は 4 報の論文で使用されています。
- Del Amo C et al. Unraveling the Signaling Secretome of Platelet-Rich Plasma: Towards a Better Understanding of Its Therapeutic Potential in Knee Osteoarthritis. J Clin Med 11:N/A (2022). PubMed: 35159924
- Del Amo C et al. Plasma-Based Bioinks for Extrusion Bioprinting of Advanced Dressings. Biomedicines 9:N/A (2021). PubMed: 34440227
- Dai B et al. lncRNA AWPPH promotes the migration and invasion of glioma cells by activating the TGF-ß pathway. Oncol Lett 18:5923-5929 (2019). PubMed: 31788066
- Yang J et al. Peptidome Analysis Reveals Novel Serum Biomarkers for Children with Autism Spectrum Disorder in China. Proteomics Clin Appl 12:e1700164 (2018). PubMed: 29754444