製品の概要

  • 製品名Human Perforin ELISA Kit (PRF1)
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    A 8 416pg/ml 20 5%
    B 8 674pg/ml 25 4%
    C 8 984pg/ml 24 3%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    A 9 580pg/ml 15 3%
    B 9 665pg/ml 31 5%
    C 9 1004pg/ml 45 5%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 40 pg/ml
  • 検出範囲
    62.5 pg/ml - 2000 pg/ml
  • 添加回収試験

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 71 % - %

  • 全工程の試験時間
    2h 00m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Perforin (PRF1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Perforin in supernatant and buffered solutions.

    A monoclonal antibody specific for Perforin has been coated onto the wells of the microtiter strips provided. Samples, including standards of known Perforin concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for Perforin are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of Perforin present in the samples. 

    This kit will recognize both endogenous and recombinant Human Perforin.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.
  • 関連疾患Familial hemophagocytic lymphohistiocytosis 2
  • 配列類似性Belongs to the complement C6/C7/C8/C9 family.
    Contains 1 C2 domain.
    Contains 1 EGF-like domain.
    Contains 1 MACPF domain.
  • ドメインThe C2 domain mediates calcium-dependent binding to lipid membranes. A subsequent conformation change leads to membrane insertion of beta-hairpin structures and pore formation. The pore is formed by transmembrane beta-strands.
  • 翻訳後修飾N-glycosylated.
  • 細胞内局在Cytoplasmic granule lumen. Secreted. Cell membrane. Endosome lumen. Stored in cytoplasmic granules of cytolytic T-lymphocytes and secreted into the cleft between T-lymphocyte and target cell. Inserts into the cell membrane of target cells and forms pores. Membrane insertion and pore formation requires a major conformation change. May be taken up via endocytosis involving clathrin-coated vesicles and accumulate in a first time in large early endosomes.
  • Information by UniProt
  • 別名
    • Cytolysin
    • FLH2
    • Lymphocyte pore-forming protein
    • P1
    • PERF_HUMAN
    • Perforin 1
    • Perforin-1
    • PFP
    • Pore forming protein
    • prf1
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab46068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human Perforin ELISA Kit (PRF1) 画像

  • Perforin measured in cell culture supernatants (tested at 1/1-1/100 dilution range; duplicates +/- SD).

  • Standard curve with background signal subtracted (duplicates; +/- SD).

  • Perforin measured in biological fluids (dilution range 1/1-1/100; duplicates +/- SD).

  • Representative standard curve using ab46068

プロトコール

Human Perforin ELISA Kit (PRF1) (ab46068) 使用論文

This product has been referenced in:
  • Freeman LM  et al. Myeloma-induced alloreactive T cells arising in myeloma-infiltrated bones include double-positive CD8+CD4+ T cells: evidence from myeloma-bearing mouse model. J Immunol 187:3987-96 (2011). Read more (PubMed: 21908738) »

See 1 Publication for this product

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Thank you for your response.   I would like to clarify that the kit batch we currently have in stock does contain standards vials 3060 pg/vial.   The following standards batch (1820 pg/vial) will be included in the next kit batch production p...

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Thanks for your new enquiry regarding ab46068 and ab46115. The quantity provided in standard vials is batch specific and it may vary from one batch to another. Regarding Perforin ELISA Kit, standard batch contains 3060 pg/vial. However, the followi...

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Thank you for your enquiry. The standard for both kits is lyophilised, thus it is not possible to provide the concentration in the vial. However, there will be a batch number-specific information/instruction printed on the label of the vial. This w...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"