製品の概要

  • 製品名
    Human PAI1 + tPA ELISA Kit
    PAI1 + tPA キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4.9%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 7.2%
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma, Tissue
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    = 0.06 ng/ml
  • 検出範囲
    0.063 ng/ml - 4 ng/ml
  • 添加回収試験

    98 %

  • 全工程の試験時間
    4h 00m
  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s PAI1 + tPA Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of PAI1 + tPA concentrations in plasma, serum, tissue extracts and cell culture supernatants.


    A PAI1 + tPA specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a PAI1 + tPA specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of PAI1 + tPA captured in plate.


    Get results in 90 minutes with Human PAI1+tPA ELISA Kit (ab192151) from our SimpleStep ELISA® range.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab108892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent dilution.

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  • Representative Standard Curve using ab108892.

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参考文献

This product has been referenced in:

See 1 Publication for this product

レビューと Q&A

Thank you for taking the time to contact us. I am sorry to hear you have some concerns regarding the results from this kit.

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I have been in contact with the lab about the possibility of using ab108892 and ab46509 with samples from Cynomolgus Monkey. Unfortunately, the cross-reactivity is low so it will not work well for monkey samples. Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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