製品の概要

  • 製品名Human PAI1 + tPA ELISA Kit
    PAI1 + tPA キット 製品一覧
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4.9%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 7.2%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma, Tissue
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    = 0.06 ng/ml
  • 検出範囲
    0.063 ng/ml - 4 ng/ml
  • 添加回収試験

    98 %

  • 全工程の試験時間
    4h 00m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s PAI1 + tPA Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of PAI1 + tPA concentrations in plasma, serum, tissue extracts and cell culture supernatants.


    A PAI1 + tPA specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a PAI1 + tPA specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of PAI1 + tPA captured in plate.


    Save time with a 90-minute ELISA protocol. Try Human PAI1+tPA ELISA Kit (ab192151) from our SimpleStep ELISA® range.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 細胞内局在Secreted
  • 別名
    • Alteplase
    • Endothelial plasminogen activator inhibitor
    • PAI
    • PAI 1
    • PAI1
    • PLANH1
    • Plasminogen activator inhibitor 1
    • Plasminogen activator tissue
    • Plasminogen activator tissue type
    • PLAT
    • Reteplase
    • Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1
    • Serpin E1
    • Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1
    • SERPINE1
    • T PA
    • T plasminogen activator
    • tissue plasminogen activator
    • Tissue type plasminogen activator
    • TPA
    see all
  • 参照データベース

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab108892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent dilution.

Human PAI1 + tPA ELISA Kit 画像

  • Representative Standard Curve using ab108892.

プロトコール

Human PAI1 + tPA ELISA Kit (ab108892) 使用論文

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