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Abcam’s PAI1 (SERPINE1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of PAI1 concentrations in Human plasma, serum, tissue extracts and cell culture supernatants.
A PAI1 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a PAI1 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of PAI1 captured in plate.
This assay recognizes both natural and recombinant Human PAI-1.
|内容||1 x 96 tests|
|100X Streptavidin-Peroxidase Conjugate||1 x 80µl|
|10X Diluent N Concentrate||1 x 30ml|
|20X Wash Buffer Concentrate||2 x 30ml|
|50X Biotinylated Human PAI1 Antibody||1 x 120µl|
|Chromogen Substrate||1 x 8ml|
|PAI1 Microplate (12 x 8 well strips)||1 unit|
|PAI1 Standard||1 vial|
|Sealing Tapes||3 units|
|Stop Solution||1 x 12ml|
Our Abpromise guarantee covers the use of ab108891 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent dilution.|
Standard curve mean of duplicates (+/- SD) with background readings subtracted
PAI1 measured in various samples showing quantity (ng) per mL of tested sample
PAI1 measured in cell lysates showing quantity (ng) per 1 million cells tested