製品の概要

  • 製品名Human PAI1 ELISA Kit (SERPINE1)
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall 4.3%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall 8.8%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma, Tissue, Cerebral Spinal Fluid
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    = 0.08 ng/ml
  • 検出範囲
    0.078 ng/ml - 5 ng/ml
  • 添加回収試験

    98 %

  • 全工程の試験時間
    4h 00m
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s PAI1 (SERPINE1) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of PAI1 concentrations in Human plasma, serum, tissue extracts and cell culture supernatants.

    A PAI1 specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a PAI1 specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of PAI1 captured in plate.

    This assay recognizes both natural and recombinant Human PAI-1.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis.
  • 組織特異性Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
  • 関連疾患Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
    Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
  • 配列類似性Belongs to the serpin family.
  • 翻訳後修飾Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
    -Met-370 bond.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • Endothelial plasminogen activator inhibitor
    • PAI
    • PAI-1
    • PAI1_HUMAN
    • Plasminogen activator inhibitor 1
    • Serpin E1
    • SERPINE1
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab108891 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent dilution.

Human PAI1 ELISA Kit (SERPINE1) 画像

  • Standard curve mean of duplicates (+/- SD) with background readings subtracted

  • PAI1 measured in various samples showing quantity (ng) per mL of tested sample

  • PAI1 measured in cell lysates showing quantity (ng) per 1 million cells tested

プロトコール

Human PAI1 ELISA Kit (SERPINE1) (ab108891) 使用論文

This product has been referenced in:
  • Giacoia EG  et al. PAI-1 leads to G1-phase cell-cycle progression through cyclin D3/cdk4/6 upregulation. Mol Cancer Res 12:322-34 (2014). Read more (PubMed: 24464915) »
  • Jin R  et al. Dietary fructose reduction improves markers of cardiovascular disease risk in Hispanic-American adolescents with NAFLD. Nutrients 6:3187-201 (2014). Human . Read more (PubMed: 25111123) »

See all 2 Publications for this product

Product Wall

Thank you for contacting us.

I can confirm that Heparin should not be interfering with the assay using these kits.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or informat...

Read More

Thank you for contacting us.

I checked with the lab and they let me know that the homology with mouse is only 2%, thus they do not recommend using it for this species. It will most likely not work.

Let me know if I can assist you f...

Read More

Thank you for contacting Abcam regarding ab108891.


Our human kit is specific for human PAI-1 total antigen measurement. It does not cross react with rabbit PAI-1. Unfortunately, we do not have a rabbit specific kit available.

Read More

Thank you for your enquiry.

This kit measures the total PAi-1 including free PAI-1, PAI-tPA complexes and PAI-1-uPA complexes.

I hope this information will be helpful.

Thank you for contacting us.

All of the kits mentioned (ab46027, ab100512, ab100640, ab108891) are suitable for use with citrate plasma.

I hope this information has been of help. If your require any further information please do not...

Read More

Thank you for contacting Abcam today.

I am sorry about the confusion over the labeling of the standards for ab108891 and ab108914. I have heard back from the lab about the correct concentrations for these standards:

ab108891 - Recon...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"