製品の概要

  • 製品名Human Oncostatin M ELISA Kit
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 1 pg/ml
  • 検出範囲
    1.37 pg/ml - 1000 pg/ml
  • 添加回収試験

    97 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 126.2 117% - 136%
    Serum 89.86 80% - 99%
    Plasma 76.63 83% - 102%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Oncostatin M Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Oncostatin M in serum, plasma, and cell culture supernatants. (Human Oncostatin M concentration is pretty low in normal serum/plasma, it may not be detected in this assay)

    This assay employs an antibody specific for Human Oncostatin M coated on a 96- well plate. Standards and samples are pipetted into the wells and Oncostatin M present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Oncostatin M antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Oncostatin M bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Growth regulator. Inhibits the proliferation of a number of tumor cell lines. Stimulates proliferation of AIDS-KS cells. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Uses both type I OSM receptor (heterodimers composed of LIPR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration.
  • 配列類似性Belongs to the LIF/OSM family.
  • 翻訳後修飾Propeptide processing is not important for receptor binding activity but may be important growth-inhibitory activity.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • MGC20461
    • ONCM_HUMAN
    • Oncostatin M precursor
    • Oncostatin-M
    • OncostatinM
    • OSM
    • OTTMUSP00000005249
    • RP23-453B22.1
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100619 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human Oncostatin M ELISA Kit 画像

  • Representative Standard Curve using ab100619.

プロトコール

Human Oncostatin M ELISA Kit (ab100619) 使用論文

This product has been referenced in:
  • Guye P  et al. Genetically engineering self-organization of human pluripotent stem cells into a liver bud-like tissue using Gata6. Nat Commun 7:10243 (2016). Read more (PubMed: 26732624) »
  • Croci DO  et al. Disrupting galectin-1 interactions with N-glycans suppresses hypoxia-driven angiogenesis and tumorigenesis in Kaposi's sarcoma. J Exp Med 209:1985-2000 (2012). Human . Read more (PubMed: 23027923) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry. I am sorry to confirm that ab100619 Oncostatin M Human ELISA Kit has only been validated for serum, pasma, cell culture spernatants, and has not been tested with CSF.  However, this ELISA kit should be accommodating to a ...

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It is very difficult for us to recommend dilutions for kits like this since samples can have a huge range in the concentration of protein present depending on the samples. The best thing to do to is to run a representative samples in a serial dilution ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"