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|Cell culture supernatant||114.9||102% - 123%|
|Plasma||86.02||79% - 94%|
Abcam’s Human NRG1 beta 1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human NRG1 beta 1 in plasma, and cell culture supernatants. (Collect plasma using EDTA or citrate as an anticoagulant. Heparinized plasma is not recommended. Human NRG1 beta 1 concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for Human NRG1 beta 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and NRG1 beta 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of NRG1 beta 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect endogenous Human NRG1 beta 1 in normal serum with ab100614, only in serum spiked with Human NRG1 beta 1.
Optimization may be required with urine samples
|内容||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|5X Assay Diluent B||1 x 15ml|
|500X HRP-Streptavidin Concentrate||1 x 200µl|
|Assay Diluent A||1 x 30ml|
|Biotinylated anti-Human NRG1 beta 1 (lyophilized)||2 vials|
|NRG1 beta 1 Microplate (12 x 8 wells)||1 unit|
|Recombinant Human NRG1 beta 1 Standard (lyophilized)||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100614 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|