製品の概要

  • 製品名Human Neurotrophin 4 ELISA Kit
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 2 pg/ml
  • 検出範囲
    1.4 pg/ml - 1000 pg/ml
  • 添加回収試験

    98 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 101.5 87% - 110%
    Serum 98.98 85% - 108%
    Plasma 95.49 83% - 107%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Neurotrophin 4 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Neurotrophin 4 in serum, plasma, and cell culture supernatants. (Human Neurotrophin 4 concentration is pretty low in normal serum/plasma, it may not be detected in this assay).

    This assay employs an antibody specific for Human Neurotrophin 4 coated on a 96-well plate. Standards and samples are pipetted into the wells and Neurotrophin 4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Neurotrophin 4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Neurotrophin 4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

法規制情報

製品の特性

  • 機能Target-derived survival factor for peripheral sensory sympathetic neurons.
  • 組織特異性Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues.
  • 関連疾患Defects in NTF4 may be associated with susceptibility to primary open angle glaucoma type 1O (GLC1O) [MIM:613100]. A form of primary open angle glaucoma (POAG). POAG is characterized by a specific pattern of optic nerve and visual field defects. The angle of the anterior chamber of the eye is open, and usually the intraocular pressure is increased. The disease is asymptomatic until the late stages, by which time significant and irreversible optic nerve damage has already taken place.
  • 配列類似性Belongs to the NGF-beta family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • GLC10
    • GLC1O
    • Neurotrophic factor 4
    • Neurotrophic factor 5
    • Neurotrophin 5
    • Neurotrophin 5 (neurotrophin 4/5)
    • Neurotrophin-4
    • Neurotrophin-5
    • Neutrophic factor 4
    • Neutrophic factor 5
    • NT 4
    • NT 4/5
    • NT 5
    • NT-4
    • NT-5
    • NT4/5
    • NT4P
    • NT5
    • NTF4
    • NTF4_HUMAN
    • NTF5
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human Neurotrophin 4 ELISA Kit 画像

  • Representative Standard Curve using ab100616

  • Representative Standard Curve using ab100616

プロトコール

Human Neurotrophin 4 ELISA Kit (ab100616) 使用論文

ab100616 has not yet been referenced specifically in any publications.

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