Human NCAM1 ELISA Kit (CD56) (ab119587)

製品の概要

  • 製品名Human NCAM1 ELISA Kit (CD56)
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 10 pg/ml
  • 検出範囲
    78 pg/ml - 5000 pg/ml
  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Human NCAM1 (CD56) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human NCAM1 (CD56) in cell culture supernatants, serum and plasma (heparin, EDTA).

    A NCAM1 (CD56) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for NCAM1 (CD56) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human NCAM1 (CD56) amount of sample captured in plate.

  • アプリケーション適用あり: ELISA, Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

法規制情報

製品の特性

  • 機能This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
  • 配列類似性Contains 2 fibronectin type-III domains.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • 細胞内局在Secreted and Cell membrane.
  • Information by UniProt
  • 別名
    • CD56
    • N-CAM-1
    • NCAM-1
    • NCAM1
    • NCAM1_HUMAN
    • Neural cell adhesion molecule 1
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab119587 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
Sandwich ELISA Use at an assay dependent concentration.

Human NCAM1 ELISA Kit (CD56) 画像

  • Representative Standard Curve using ab119587.

プロトコール

Human NCAM1 ELISA Kit (CD56) (ab119587) 使用論文

ab119587 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your interest in the CD44 and CD56 ELISA kits, ab45912 and ab119587. Neither has been tested for reactivity with mouse or rat samples. We are unable to assess the probability of cross-reaction by BLAST, as the locations of the epitopes th...

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Abreview for CD56 Human ELISA Kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews

Sample: Human Serum (Heparin plasma)
Specification: Heparin plasma
Amount of Sample: 1 uL (x100 dilution) µg
Type of Kit Used: ELISA
Positive control: Kit standards
Did you use the Abcam Kit protocol?: Yes
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投稿 Sep 12 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"