製品の概要

  • 製品名Human NBL1 ELISA Kit
    NBL1 キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 35 pg/ml
  • 検出範囲
    27.43 pg/ml - 20000 pg/ml
  • 添加回収試験

    95 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 111.3 107% - 122%
    Serum 100.1 91% - 109%
    Plasma 81.82 72% - 90%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s NBL1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human NBL1 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human NBL1 coated on a 96-well plate. Standards and samples are pipetted into the wells and NBL1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human NBL1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of NBL1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm..

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Possible candidate as a tumor suppressor gene of neuroblastoma. May play an important role in preventing cells from entering the final stage (G1/S) of the transformation process.
  • 組織特異性Most abundant in normal lung and meningioma.
  • 配列類似性Belongs to the DAN family.
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • DAN
    • DAN domain family member 1
    • DAND 1
    • DAND1
    • Differential screening selected gene aberrant in neuroblastoma
    • MGC8972
    • NB
    • NBL 1
    • NBL1
    • NBL1_HUMAN
    • Neuroblastoma candidate region suppression of tumorigenicity 1
    • Neuroblastoma suppression of tumorigenicity 1
    • Neuroblastoma suppressor of tumorigenicity 1
    • NO 3
    • NO3
    • Protein N03
    • Zinc finger protein DAN
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab99997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human NBL1 ELISA Kit 画像

  • Representative Standard Curve using ab99997

  • Representative Standard Curve using ab99997

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Human NBL1 ELISA Kit (ab99997) 使用論文

ab99997 has not yet been referenced specifically in any publications.

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