製品の概要

  • 製品名Human Myoglobin ELISA Kit
    Myoglobin キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Serum
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    = 5 ng/ml
  • 検出範囲
    5 ng/ml - 1000 ng/ml
  • 添加回収試験

    102.8 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Serum 102.8 39ng/ml - 780ng/ml

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s Myoglobin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Myoglobin in Human serum.

    A 96-well plate has been precoated with anti-Myoglobin antibodies. Samples and standards and are added to the wells, where Myoglobin in the sample and standards binds to the precoated antibody. Added anti-Myoglobin HRP conjugate binds to this antibody-Myoglobin complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of Myoglobin in the sample and the intensity is measured at 450 nm.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108652 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human Myoglobin ELISA Kit 画像

  • Representative standard curve using ab108652

プロトコール

Human Myoglobin ELISA Kit (ab108652) 使用論文

This product has been referenced in:
  • Naclerio F  et al. A multi-ingredient containing carbohydrate, proteins L-glutamine and L-carnitine attenuates fatigue perception with no effect on performance, muscle damage or immunity in soccer players. PLoS One 10:e0125188 (2015). Human . Read more (PubMed: 25915424) »

See 1 Publication for this product

Product Wall

Thank you for contacting us. The kit is for one 96 well plate, so taking into account 5 standards and a blank, plus doing everything in duplicate allows you to run 42 unique samples.


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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"