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|Cell culture supernatant||95.44||84% - 103%|
|Serum||94.75||83% - 103%|
|Plasma||97.13||85% - 104%|
Abcam’s MMP8 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MMP8 in serum, plasma, and cell culture supernatants. (Collect plasma using heparin as an anticoagulant. EDTA and Citrate are not recommended).
This assay employs an antibody specific for Human MMP8 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP8 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MMP8 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP8 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimisation may be required with urine samples.
|内容||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|5X Assay Diluent||1 x 15ml|
|600X HRP-Streptavidin Concentrate||1 x 200µl|
|Biotinylated anti-Human MMP8||2 vials|
|MMP8 Microplate (12 x 8 wells)||1 unit|
|Recombinant Human MMP8 Standard||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100609 in the following tested applications.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab100609 has not yet been referenced specifically in any publications.