製品の概要

  • 製品名Human MMP7 ELISA Kit
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 0.15 ng/ml
  • 検出範囲
    0.137 ng/ml - 100 ng/ml
  • 添加回収試験

    90 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 90.52 82% - 102%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s MMP7 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MMP7 in cell culture supernates.

    This assay employs an antibody specific for Human MMP7 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MMP7 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase.
  • 配列類似性Belongs to the peptidase M10A family.
  • ドメインThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 細胞内局在Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 別名
    • Matrilysin
    • Matrin
    • Matrix metalloproteinase 7
    • Matrix metalloproteinase-7
    • MMP-7
    • MMP7
    • MMP7_HUMAN
    • MPSL1
    • Pump 1 protease
    • Pump-1 protease
    • PUMP1
    • Uterine metalloproteinase
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100608 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human MMP7 ELISA Kit 画像

  • Representative Standard Curve using ab100608.

プロトコール

Human MMP7 ELISA Kit (ab100608) 使用論文

ab100608 has not yet been referenced specifically in any publications.

Product Wall

I can confirm that kits ab100606 and ab100608 recognizes both pro and mature forms of respective proteins.

I now have further information to share from the lab in regards to the MMP7 Human ELISA Kit (ab100608).

The lab have further confirmed that the results obtained with the lot numbers GR88563 & GR98815 have performed as expected: standard cu...

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I am sorry for the delay in getting back to you. I now have some further input form the lab regarding the MMP7 Human ELISA Kit (ab100608) your customer has been having some difficulty with.

The first lot tested does contain good linearity and...

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Thank you for contacting us.

I am sorry to hear your customer has been experiencing difficulties with the MMP7 Human ELISA Kit (ab100608). We take product complaints very seriously, and investigate every product that we feel may not be perfo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"