製品の概要

  • 製品名Human MMP13 ELISA Kit
  • 検出方法Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall < 10%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall < 12%
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 6 pg/ml
  • 検出範囲
    8.23 pg/ml - 6000 pg/ml
  • 添加回収試験

    93 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 94.72 84% - 103%
    Serum 91.99 82% - 102%
    Plasma 93.13 83% - 103%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s MMP13 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of Human MMP13 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human MMP13 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP13 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MMP13 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP13 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

    The kit detects total MMP-13 levels.

  • 特記事項

    Optimisation may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process.
  • 組織特異性Seems to be specific to breast carcinomas.
  • 関連疾患Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia Missouri type (SEMD-MO) [MIM:602111]. A bone disease characterized by moderate to severe metaphyseal changes, mild epiphyseal involvement, rhizomelic shortening of the lower limbs with bowing of the femora and/or tibiae, coxa vara, genu varum and pear-shaped vertebrae in childhood. Epimetaphyseal changes improve with age.
    Defects in MMP13 are the cause of metaphyseal anadysplasia type 1 (MANDP1) [MIM:602111]. Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia.
  • 配列類似性Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • ドメインThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 細胞内局在Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 別名
    • CLG 3
    • CLG3
    • Collagenase 3
    • Collagenase3
    • MANDP1
    • Matrix metallopeptidase 13 (collagenase 3)
    • Matrix Metalloproteinase 13
    • Matrix metalloproteinase-13
    • MMP 13
    • MMP-13
    • Mmp13
    • MMP13_HUMAN
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100605 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human MMP13 ELISA Kit 画像

  • MMP13 measured in cell supernatanses showing quantity (pg) per mL of tested sample

  • Representative standard curve using ab100605

プロトコール

Human MMP13 ELISA Kit (ab100605) 使用論文

This product has been referenced in:
  • Collins JA  et al. Oxygen and pH-sensitivity of human osteoarthritic chondrocytes in 3-D alginate bead culture system. Osteoarthritis Cartilage 21:1790-8 (2013). ELISA ; Human . Read more (PubMed: 23850530) »
  • Jayasuriya CT  et al. Matrilin-3 Induction of IL-1 receptor antagonist Is required for up-regulating collagen II and aggrecan and down-regulating ADAMTS-5 gene expression. Arthritis Res Ther 14:R197 (2012). ELISA . Read more (PubMed: 22967398) »

See all 2 Publications for this product

Product Wall

Abreviews
I tested this kit for cross-reactivity with bovine and porcine MMP-13 using bovine standards and a porcine positive control sample from a USBio bovine MMP-13 ELISA kit. The kit did not detect bovine or porcine samples (very low or negative OD values).
The human standards seemed to work well, and the standard diluent also seems to be important because human standards in the bovine diluent produced much lower signals.
Username

Ms. Ann Ouyang

Verified customer

投稿 Apr 08 2015

For plasma:
Collect blood into EDTA or Sodium heparin tube (e.g. BD vacutainer, Cat. No: 8001302 or 16852)

Spin 10 minutes at 3000 rpm

Aliquot into small tubes and store at –800C until use.

For serum:

Colle...

Read More

Thank you for contacting Abcam. I have contacted the lab and they have confirmed that the MMP13 Human ELISA kit (ab100605) recognizes total MMP-13 levels. If there is anything else that I can do, please do not hesitate to contact me.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"