製品の概要

  • 製品名

    Human MMP13 ELISA Kit


    MMP13 キット 製品一覧
  • 検出方法
    Colorimetric
  • 再現性
    Intra-Assay(同時再現性)
    サンプル N 平均値 SD CV%
    Overall < 10%
    Inter-Assay(日差再現性)
    サンプル N 平均値 SD CV%
    Overall < 12%
  • サンプルの種類
    Cell culture supernatant, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 6 pg/ml
  • 検出範囲
    8.23 pg/ml - 6000 pg/ml
  • 添加回収試験

    93 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 94.72 84% - 103%
    Plasma 93.13 83% - 103%

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s MMP13 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme linked immunosorbent assay for the quantitative measurement of Human MMP13 in plasma and cell culture supernatants.


    This assay employs an antibody specific for Human MMP13 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP13 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MMP13 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP13 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    The kit detects total MMP-13 levels.


    We have not been able to detect endogenous Human MMP13 in normal serum with ab100605, only in serum spiked with Human MMP13.

  • 特記事項

    Optimisation may be required with urine samples.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

法規制情報

製品の特性

  • 保存方法
    Store at -20°C. Please refer to protocols.
  • 内容 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent 1 x 15ml
    700X HRP-Streptavidin Concentrate 1 x 200µl
    Biotinylated anti-Human MMP13 (lyophilized) 2 vials
    MMP13 Microplate (12 x 8 wells) 1 unit
    Recombinant Human MMP13 Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • 研究分野
  • 機能
    Degrades collagen type I. Does not act on gelatin or casein. Could have a role in tumoral process.
  • 組織特異性
    Seems to be specific to breast carcinomas.
  • 関連疾患
    Defects in MMP13 are the cause of spondyloepimetaphyseal dysplasia Missouri type (SEMD-MO) [MIM:602111]. A bone disease characterized by moderate to severe metaphyseal changes, mild epiphyseal involvement, rhizomelic shortening of the lower limbs with bowing of the femora and/or tibiae, coxa vara, genu varum and pear-shaped vertebrae in childhood. Epimetaphyseal changes improve with age.
    Defects in MMP13 are the cause of metaphyseal anadysplasia type 1 (MANDP1) [MIM:602111]. Metaphyseal anadysplasia consists of an abnormal bone development characterized by severe skeletal changes that, in contrast with the progressive course of most other skeletal dysplasias, resolve spontaneously with age. Clinical characteristics are evident from the first months of life and include slight shortness of stature and a mild varus deformity of the legs. Patients attain a normal stature in adolescence and show improvement or complete resolution of varus deformity of the legs and rhizomelic micromelia.
  • 配列類似性
    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • ドメイン
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 細胞内局在
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 別名
    • CLG 3
    • CLG3
    • Collagenase 3
    • Collagenase3
    • MANDP1
    • Matrix metallopeptidase 13 (collagenase 3)
    • Matrix Metalloproteinase 13
    • Matrix metalloproteinase-13
    • MMP 13
    • MMP-13
    • Mmp13
    • MMP13_HUMAN
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100605 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • MMP13 measured in cell supernatanses showing quantity (pg) per mL of tested sample

  • Representative standard curve using ab100605

プロトコール

参考文献

This product has been referenced in:
  • Alexander K  et al. Levels of Matrix Metalloproteinases in Arthroplasty Patients and Their Correlation With Inflammatory and Thrombotic Activation Processes. Clin Appl Thromb Hemost 22:441-6 (2016). Read more (PubMed: 27052781) »
  • Zeng JZ  et al. Increased receptor activator of nuclear factor ?ß ligand/osteoprotegerin ratio exacerbates cartilage destruction in osteoarthritis in vitro. Exp Ther Med 12:2778-2782 (2016). Read more (PubMed: 27698783) »

See all 4 Publications for this product

レビューと Q&A

Abreviews
I tested this kit for cross-reactivity with bovine and porcine MMP-13 using bovine standards and a porcine positive control sample from a USBio bovine MMP-13 ELISA kit. The kit did not detect bovine or porcine samples (very low or negative OD values).
The human standards seemed to work well, and the standard diluent also seems to be important because human standards in the bovine diluent produced much lower signals.
Username

Ms. Ann Ouyang

Verified customer

投稿 Apr 08 2015

For plasma:
Collect blood into EDTA or Sodium heparin tube (e.g. BD vacutainer, Cat. No: 8001302 or 16852)

Spin 10 minutes at 3000 rpm

Aliquot into small tubes and store at –800C until use.

For serum:

Colle...

Read More

Thank you for contacting Abcam. I have contacted the lab and they have confirmed that the MMP13 Human ELISA kit (ab100605) recognizes total MMP-13 levels. If there is anything else that I can do, please do not hesitate to contact me.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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