製品の概要

  • 製品名Human MMP1 ELISA Kit
    MMP1 キット 製品一覧
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 8 pg/ml
  • 検出範囲
    24.69 pg/ml - 18000 pg/ml
  • 添加回収試験

    98 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 98.66 90% - 106%
    Serum 97.78 88% - 106%
    Plasma 99.59 90% - 107%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 製品の概要

    Abcam’s MMP1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MMP1 pro and active forms in serum, plasma (using heparin as an anticoagulant, EDTA and Citrate are not recommended), and cell culture supernatants.

    This assay employs an antibody specific for Human MMP1 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MMP1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimisation may be required with urine samples

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.
  • 配列類似性Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • ドメインThere are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • 翻訳後修飾Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.
  • 細胞内局在Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • 別名
    • 27 kDa interstitial collagenase
    • CLG
    • CLGN
    • Fibroblast collagenase
    • Interstitial collagenase
    • Matrix metallopeptidase 1 (interstitial collagenase)
    • Matrix Metalloproteinase 1
    • Matrix metalloproteinase-1
    • MMP 1
    • MMP-1
    • MMP1
    • MMP1_HUMAN
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100603 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human MMP1 ELISA Kit 画像

  • MMP1 measured in cell culture medium with background signal subtracted (duplicates +/- SD).

  • MMP1 measured in biological fluids with background signal subtracted (duplicates +/- SD).

  • Representative Standard Curve using ab100603.

プロトコール

Human MMP1 ELISA Kit (ab100603) 使用論文

This product has been referenced in:
  • Dey S  et al. Matrix metalloproteinase-1 (MMP-1) Promoter polymorphisms are well linked with lower stomach tumor formation in eastern Indian population. PLoS One 9:e88040 (2014). Read more (PubMed: 24505369) »

See 1 Publication for this product

Product Wall

Nous ne recommandons pas de modifier les concentrations des courbes standards mais plutôt de diluer vos échantillons.

Toutefois, si vous voulez vraiment changer les courbes standards ceci est possible. Cependant en augmentant les ...

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Thank you for your inquiry.

For ab100603 lot GR47531-1, it was entered into stock in June 2011. We set an expiration guidelinefor 1 year from that point, so June 2012.

For ab100651 lot GR49880-1 was put into stock in July, Aug and O...

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Thank you and your customerfor taking the time to complete our questionnaire and contact us. I am sorry to hear your customerhas had difficulty obtaining satisfactory results from this antibody.

The details your customerhas kindly provided w...

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Thank you for contacting Abcam.

Unfortunately, to our knowledge ab100603, ab100607, and ab100605 have not been tested with monkey samples so there is no cross reactivity data available. However, I believe that MMPs are very well conserved amo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"