製品の概要

  • 製品名Human MIP5 ELISA Kit (CCL15)
  • 検出方法Colorimetric
  • テスト
    1 x 96 well plate
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプSandwich (quantitative)
  • 検出感度
    < 3 pg/ml
  • 検出範囲
    4.1 pg/ml - 1000 pg/ml
  • 添加回収試験

    100 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 101.5 91% - 112%
    Serum 105.3 92% - 115%
    Plasma 98.23 90% - 114%

  • ステップMultiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s MIP5 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MIP5 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human MIP5 coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human MIP5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション適用あり: Sandwich ELISAmore details
  • 試験プラットフォームMicroplate

製品の特性

  • 機能Chemotactic factor that attracts T-cells and monocytes, but not neutrophils, eosinophils, or B-cells. Acts mainly via CC chemokine receptor CCR1. Also binds to CCR3. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are more potent chemoattractants than the small-inducible cytokine A15.
  • 組織特異性Most abundant in heart, skeletal muscle and adrenal gland. Lower levels in placenta, liver, pancreas and bone marrow. CCL15(22-92), CCL15(25-92) and CCL15(29-92) are found in high levels in synovial fluids from rheumatoid patients.
  • 配列類似性Belongs to the intercrine beta (chemokine CC) family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 別名
    • C-C motif chemokine 15
    • CC chemokine 3
    • CCL15
    • CCL15(29-92)
    • CCL15_HUMAN
    • Chemokine (C C motif) ligand 15
    • Chemokine CC-2
    • Chemokine CC2
    • HCC-2
    • HCC2
    • HMRP 2B
    • HMRP2B
    • Leukotactin 1
    • Leukotactin-1
    • LKN-1
    • LKN1
    • Macrophage inflammatory protein 5
    • MIP 1D
    • MIP-1 delta
    • MIP-1D
    • MIP-5
    • MIP1 delta
    • MIP1d
    • MIP5
    • MRP 2B
    • Mrp-2b
    • Mrp2b
    • NCC-3
    • NCC3
    • New CC chemokine 3
    • OTTHUMP00000163955
    • SCYA15
    • SCYL3
    • Small inducible cytokine A15
    • Small inducible cytokine subfamily A (Cys Cys), member 15
    • Small-inducible cytokine A15
    • SY15
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

Human MIP5 ELISA Kit (CCL15) 画像

  • Representative Standard Curve using ab100598.

  • Representative Standard Curve using ab100598.

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Human MIP5 ELISA Kit (CCL15) (ab100598) 使用論文

ab100598 has not yet been referenced specifically in any publications.

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