製品の概要

  • 製品名
    Human MIP4 ELISA Kit (CCL18)
  • 検出方法
    Colorimetric
  • サンプルの種類
    Cell culture supernatant, Serum, Plasma
  • アッセイタイプ
    Sandwich (quantitative)
  • 検出感度
    < 2 pg/ml
  • 検出範囲
    2.74 pg/ml - 2000 pg/ml
  • 添加回収試験

    96 %

    特定サンプルでの回収試験
    サンプルの種類 平均 % 測定範囲
    Cell culture supernatant 98.61 86% - 105%
    Serum 95.74 83% - 103%
    Plasma 96.53 84% - 104%

  • ステップ
    Multiple steps standard assay
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam’s MIP4 (CCL18) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human MIP4 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human MIP4 coated on a 96-well plate. Standards and samples are pipetted into the wells and MIP4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated antiHuman MIP4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MIP4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • 特記事項

    Optimization may be required with urine samples.

  • アプリケーション
    適用あり: Sandwich ELISAmore details
  • 試験プラットフォーム
    Microplate

法規制情報

製品の特性

  • 保存方法
    Store at -20°C. Please refer to protocols.
  • 内容 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    600X HRP-Streptavidin Concentrate 1 x 200µl
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human MIP4 (lyophilized) 2 vials
    MIP4 Microplate (12 x 8 wells) 1 unit
    Recombinant Human MIP4 Standard 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Reagent 1 x 12ml
  • 研究分野
  • 機能
    Chemotactic factor that attracts lymphocytes but not monocytes or granulocytes. May be involved in B-cell migration into B-cell follicles in lymph nodes. Attracts naive T-lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T-cells, CD4+ and CD8+ T-cells and thus may play a role in both humoral and cell-mediated immunity responses.
  • 組織特異性
    Expressed at high levels in lung, lymph nodes, placenta, bone marrow, dendritic cells present in germinal centers and T-cell areas of secondary lymphoid organs and macrophages derived from peripheral blood monocytes. Not expressed by peripheral blood monocytes and a monocyte-to-macrophage differentiation is a prerequisite for expression. Expressed in synovial fluids from patients with rheumatoid and septic arthritis and in ovarian carcinoma ascitic fluid.
  • 配列類似性
    Belongs to the intercrine beta (chemokine CC) family.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 別名
    • Alternative macrophage activation associated CC chemokine 1
    • Alternative macrophage activation-associated CC chemokine 1
    • AMAC 1
    • AMAC-1
    • AMAC1
    • C C motif chemokine 18
    • CC chemokine ligand 18
    • CC chemokine PARC
    • CCL18
    • CCL18(4-69)
    • CCL18_HUMAN
    • Chemokine (C C motif) ligand 18
    • Chemokine (C C) dendritic
    • Chemokine ligand 18
    • CKb7
    • DC CK1
    • DC-CK1
    • DCCK1
    • Dendritic cell chemokine 1
    • Macrophage inflammatory protein 4
    • MIP 4
    • MIP-4
    • MIP4
    • PARC
    • Pulmonary and activation regulated chemokine
    • Pulmonary and activation-regulated chemokine
    • Small inducible cytokine A18
    • Small-inducible cytokine A18
    see all
  • 参照データベース

アプリケーション

Our Abpromise guarantee covers the use of ab100620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Sandwich ELISA Use at an assay dependent concentration.

画像

  • MIP4 measured in human biological fluids; signals in rat and mouse samples were below level of detection (duplicates +/- SD).

  • Data shows secretion of MIP4 (CCL18) by human macrophages isolated by negative selection in culture for 3 days.
    Specificity shown by selective secretion by M2a macrophages (MSCF + IL4) and M2c (MCSF + IL10) compared to the others subtypes (M0 = MCSF, M1 = MSCF + IFNg, DEX = MCSF + Dexamethasone, TGFb = MCSF + TGFb).

  • Representative Standard Curve using ab100620.

  • Representative Standard Curve using ab100620.

プロトコール

参考文献

This product has been referenced in:
  • Muñoz-Esquerre M  et al. Vascular disease in COPD: Systemic and pulmonary expression of PARC (Pulmonary and Activation-Regulated Chemokine). PLoS One 12:e0177218 (2017). Read more (PubMed: 28545096) »
  • Ting KR  et al. Novel panel of protein biomarkers to predict response to bortezomib-containing induction regimens in multiple myeloma patients. BBA Clin 8:28-34 (2017). Read more (PubMed: 28725572) »

See all 4 Publications for this product

レビューと Q&A

The Assay Diluent A will have a slight yellow haze colorization.

CCL18-MIP4 secretion by macrophages

Excellent Excellent 5/5 (Ease of Use)
Abreviews
CCL18-MIP4 secretion by human macrophages isolated by negative selection in culture for 3 days.
Kit easy to use, the assay takes about 5.30hr, excellent specificity as shown by selective secretion by M2a macrophages (MSCF+IL4) & M2c (MCSF+IL10) compared to the others subtypes (M0=MCSF M1=MSCF+IFNg DEX=MCSF+Dexamethasone TGFb=MCSF+TGFb)
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Verified customer

投稿 Mar 18 2014

Thank you for contacting us.

We unfortunately do not have these kits available for human samples. I however have contacted the product manager and asked them if these products are in pipeline, I will get back to you once I get it confirmed. Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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